ArticleEukaryotic cell2012
The Tlo proteins are stoichiometric components of Candida albicans mediator anchored via the Med3 subunit.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Paralogs of thebioRxiv : the preprint server for biology · 2026Article
- Fluconazole resistance andMicrobiology (Reading, England) · 2025Article
- Functional genomic analysis of genes important for Candida albicans fitness in diverse environmental conditions.Cell reports · 2024Article
- Med3-mediated NADPH generation to helpApplied and environmental microbiology · 2024Article
- Identification of an active RNAi pathway inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Deletion of the Candida albicans TLO gene family using CRISPR-Cas9 mutagenesis allows characterisation of functional differences in α-, β- and γ- TLO gene function.PLoS genetics · 2023Article
- The role of the Mediator complex in fungal pathogenesis and response to antifungal agents.Essays in biochemistry · 2023Review
- Multifactor transcriptional control of alternative oxidase induction integrates diverse environmental inputs to enable fungal virulence.Nature communications · 2023Article
- Architectural groups of a subtelomeric gene family evolve along distinct paths in Candida albicans.G3 (Bethesda, Md.) · 2022Article
- Stress combined with loss of the Candida albicans SUMO protease Ulp2 triggers selection of aneuploidy via a two-step process.PLoS genetics · 2022Article
- Review
- Electron cryomicroscopy observation of acyl carrier protein translocation in type I fungal fatty acid synthase.Scientific reports · 2019Article
- Role of Mediator in virulence and antifungal drug resistance in pathogenic fungi.Current genetics · 2019Review
- Protein-Protein Interactions inFrontiers in microbiology · 2019Review
- Tobramycin reduces key virulence determinants in the proteome of Pseudomonas aeruginosa outer membrane vesicles.PloS one · 2019Article
- Candida glabrata Med3 Plays a Role in Altering Cell Size and Budding Index To Coordinate Cell Growth.Applied and environmental microbiology · 2018Article
- Functional diversification accompanies gene family expansion of MED2 homologs in Candida albicans.PLoS genetics · 2018Article
- Article
- Candida albicans Swi/Snf and Mediator Complexes Differentially Regulate Mrr1-InducedAntimicrobial agents and chemotherapy · 2017Article
- Mediator Tail Module Is Required for Tac1-ActivatedAntimicrobial agents and chemotherapy · 2017Article
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6 authors.
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Abstract
The amplification of the TLO (for telomere-associated) genes in Candida albicans, compared to its less pathogenic, close relative Candida dubliniensis, suggests a role in virulence. Little, however, is known about the function of the Tlo proteins. We have purified the Mediator coactivator complex from C. albicans (caMediator) and found that Tlo proteins are a stoichiometric component of caMediator. Many members of the Tlo family are expressed, and each is a unique member of caMediator. Protein expression analysis of individual Tlo proteins, as well as the purification of tagged Tlo proteins, demonstrate that there is a large free population of Tlo proteins in addition to the Mediator-associated population. Coexpression and copurification of Tloα12 and caMed3 in Escherichia coli established a direct physical interaction between the two proteins. We have also made a C. albicans med3Δ/Δ strain and purified an intact Mediator from this strain. The analysis of the composition of the med3Δ Mediator shows that it lacks a Tlo subunit. Regarding Mediator function, the med3Δ/Δ strain serves as a substitute for the difficult-to-make tloΔ/Δ C. albicans strain. A potential role of the TLO and MED3 genes in virulence is supported by the inability of the med3Δ/Δ strain to form normal germ tubes. This study of caMediator structure provides initial clues to the mechanism of action of the Tlo genes and a platform for further mechanistic studies of caMediator's involvement in gene regulatory patterns that underlie pathogenesis.
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