ArticleEukaryotic cell2012
A cytochrome b5-containing plastid-located fatty acid desaturase from Chlamydomonas reinhardtii.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 79 citations in OpenAlex.
- Mechanisms of cold adaptation in a novel marine eukaryotic phytoplankton-psychrophilic microalga Chlamydomonas sp. LWD-1.Plant cell reports · 2026Article
- Regulatory network rewiring drives strain-specific lipid accumulation response in Chlorella sorokiniana under nutrient starvation.The Plant journal : for cell and molecular biology · 2026Article
- Biological and Nutritional Applications of Microalgae.Nutrients · 2024Review
- Inhibition of monogalactosyldiacylglycerol synthesis by down-regulation of MGD1 leads to membrane lipid remodeling and enhanced triacylglycerol biosynthesis in Chlamydomonas reinhardtii.Biotechnology for biofuels and bioproducts · 2022Article
- An Overview of the Electron-Transfer Proteins That Activate Alkane Monooxygenase (AlkB).Frontiers in microbiology · 2022Review
- Modification of sesame (Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- Characterization of fatty acid desaturases reveals stress-induced synthesis of C18 unsaturated fatty acids enriched in triacylglycerol in the oleaginous alga Chromochloris zofingiensis.Biotechnology for biofuels · 2021Article
- Large fluxes of fatty acids from membranes to triacylglycerol and back during N-deprivation and recovery in Chlamydomonas.Plant physiology · 2021Article
- In Silico Analysis of Partial Fatty Acid Desaturase 2 cDNA FromBioinformatics and biology insights · 2021Article
- Plastidic Δ6 Fatty-Acid Desaturases with Distinctive Substrate Specificity Regulate the Pool of C18-PUFAs in the Ancestral PicoalgaPlant physiology · 2020Article
- Endoplasmic reticulum retention signaling and transmembrane channel proteins predicted for oilseed ω3 fatty acid desaturase 3 (FAD3) genes.Functional & integrative genomics · 2020Article
- Chlamydomonas CHT7 Is Required for an Effective Quiescent State by Regulating Nutrient-Responsive Cell Cycle Gene Expression.The Plant cell · 2020Article
- Potential and Challenges of Improving Photosynthesis in Algae.Plants (Basel, Switzerland) · 2020Review
- Chlorella vulgaris genome assembly and annotation reveals the molecular basis for metabolic acclimation to high light conditions.The Plant journal : for cell and molecular biology · 2019Article
- Subcellular Energetics and Carbon Storage inCells · 2019Review
- Glycerolipid Characterization and Nutrient Deprivation-Associated Changes in the Green PicoalgaPlant physiology · 2017Article
- Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker.Plant molecular biology · 2016Article
- Classification and substrate head-group specificity of membrane fatty acid desaturases.Computational and structural biotechnology journal · 2016Article
- Progress and Challenges in Microalgal Biodiesel Production.Frontiers in microbiology · 2016Review
- Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae.Frontiers in plant science · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monogalactosyldiacylglycerol (MGDG) in Chlamydomonas reinhardtii and other green algae contains hexadeca-4,7,10,13-tetraenoic acid (16:4) in the glycerol sn-2 position. While many genes necessary for the introduction of acyl chain double bonds have been functionally characterized, the Δ4-desaturase remained unknown. Using a phylogenetic comparison, a candidate gene encoding the MGDG-specific Δ4-desaturase from Chlamydomonas (CrΔ4FAD) was identified. CrΔ4FAD shows all characteristic features of a membrane-bound desaturase, including three histidine boxes and a transit peptide for chloroplast targeting. But it also has an N-terminal cytochrome b(5) domain, distinguishing it from other known plastid desaturases. Cytochrome b(5) is the primary electron donor for endoplasmic reticulum (ER) desaturases and is often fused to the desaturase domain in desaturases modifying the carboxyl end of the acyl group. Difference absorbance spectra of the recombinant cytochrome b(5) domain of CrΔ4FAD showed that it is functional in vitro. Green fluorescent protein fusions of CrΔ4FAD localized to the plastid envelope in Chlamydomonas. Interestingly, overproduction of CrΔ4FAD in Chlamydomonas not only increased levels of 16:4 acyl groups in cell extracts but specifically increased the total amount of MGDG. Vice versa, the amount of MGDG was lowered in lines with reduced levels of CrΔ4FAD. These data suggest a link between MGDG molecular species composition and galactolipid abundance in the alga, as well as a specific function for this fatty acid in MGDG.
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