ArticleEukaryotic cell2012
Chitin-like molecules associate with Cryptococcus neoformans glucuronoxylomannan to form a glycan complex with previously unknown properties.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
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- Cell wall melanin impedes growth of theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Screening of the Pandemic Response Box Reveals an Association between Antifungal Effects of MMV1593537 and the Cell Wall ofMicrobiology spectrum · 2022Article
- Monoclonal Antibodies against Cell Wall Chitooligomers as Accessory Tools for the Control of Cryptococcosis.Antimicrobial agents and chemotherapy · 2021Article
- Fungal Extracellular Vesicles in Pathophysiology.Sub-cellular biochemistry · 2021Article
- Ultrastructural Study ofFrontiers in microbiology · 2021Article
- Article
- The capsule ofVirulence · 2019Review
- Article
- Unraveling synthesis of the cryptococcal cell wall and capsule.Glycobiology · 2018Review
- Lack of chitin synthase genes impacts capsular architecture and cellular physiology inCell surface (Amsterdam, Netherlands) · 2018Article
- Immune Recognition of Fungal Polysaccharides.Journal of fungi (Basel, Switzerland) · 2017Review
- Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection.Scientific reports · 2016Article
- Extracellular Vesicle-Associated Transitory Cell Wall Components and Their Impact on the Interaction of Fungi with Host Cells.Frontiers in microbiology · 2016Review
- Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates.Scientific reports · 2015Article
- Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans.Eukaryotic cell · 2014Article
- Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct.Future microbiology · 2014Article
- Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis.Fungal genetics and biology : FG & B · 2013Article
- A Paracoccidioides brasiliensis glycan shares serologic and functional properties with cryptococcal glucuronoxylomannan.Fungal genetics and biology : FG & B · 2012Article
- In good company: association between fungal glycans generates molecular complexes with unique functions.Frontiers in microbiology · 2012Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
In prior studies, we demonstrated that glucuronoxylomannan (GXM), the major capsular polysaccharide of the fungal pathogen Cryptococcus neoformans, interacts with chitin oligomers at the cell wall-capsule interface. The structural determinants regulating these carbohydrate-carbohydrate interactions, as well as the functions of these structures, have remained unknown. In this study, we demonstrate that glycan complexes composed of chitooligomers and GXM are formed during fungal growth and macrophage infection by C. neoformans. To investigate the required determinants for the assembly of chitin-GXM complexes, we developed a quantitative scanning electron microscopy-based method using different polysaccharide samples as inhibitors of the interaction of chitin with GXM. This assay revealed that chitin-GXM association involves noncovalent bonds and large GXM fibers and depends on the N-acetyl amino group of chitin. Carboxyl and O-acetyl groups of GXM are not required for polysaccharide-polysaccharide interactions. Glycan complex structures composed of cryptococcal GXM and chitin-derived oligomers were tested for their ability to induce pulmonary cytokines in mice. They were significantly more efficient than either GXM or chitin oligomers alone in inducing the production of lung interleukin 10 (IL-10), IL-17, and tumor necrosis factor alpha (TNF-α). These results indicate that association of chitin-derived structures with GXM through their N-acetyl amino groups generates glycan complexes with previously unknown properties.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.