Evidence map›Paper›PMID 22561071›Full record

ArticleMolecular and biochemical parasitology2012

Transient and stable transfection in the protozoan parasite Entamoeba invadens.

Gretchen M Ehrenkaufer, Upinder Singh

Abstract read
In one paragraph

Article in Molecular and biochemical parasitology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
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  3. Article
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  5. Article
  6. Article
  7. The NADFrontiers in cellular and infection microbiology · 2020
    Article
  8. Article
  9. An NADeLife · 2018
    Article
  10. High-Throughput Screening ofFrontiers in cellular and infection microbiology · 2018
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Gretchen M EhrenkauferDivision of Infectious Diseases, Department of Internal Medicine, Stanford University, Stanford, CA 94305-5107, USA.
Upinder Singh

Funding

Transcriptome analysis of Entamoeba development using RNA-sequencingR21AI094887 · NIAID · STANFORD UNIVERSITY · PI SINGH, UPINDER · 2011 to 2012
$435k
Developing tools for genetic manipulation in Entamoeba invadensR03AI088042 · NIAID · STANFORD UNIVERSITY · PI SINGH, UPINDER · 2010 to 2011
$159k
NIAID NIH HHS AI088042NIAID NIH HHS AI094887NIAID NIH HHS R03 AI088042NIAID NIH HHS R21 AI094887
6 · The paper itself

Abstract

Entamoeba histolytica is an important human pathogen and a major health problem worldwide. Many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in E. histolytica. The reptile parasite Entamoeba invadens is a vital model system for studying stage conversion since it can be induced to undergo both encystation and excystation with high efficiency in vitro. However, functional studies using E. invadens have been limited by the lack of genetic tools in this species. Here, we report a new method for both transient and stable transfection of E. invadens. These new tools will greatly enhance research into Entamoeba development.

Indexed as

EntamoebaSpores, ProtozoanTransfection

Identifiers

PMID22561071
PMCPMC3358517

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.