Evidence map›Paper›PMID 22561054›Full record

ArticleGastroenterology2012

Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets.

Takeshi Ohki, Masayuki Yamato, Masaho Ota, Ryo Takagi, Daisuke Murakami, Makoto Kondo, Ryo Sasaki, Hideo Namiki, Teruo Okano, Masakazu Yamamoto

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In one paragraph

Article in Gastroenterology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 202 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
202citing papers in PubMed, 3 pooled it
39.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

202 citing papers in PubMed, 3 syntheses or guidelines pooled it, 515 citations in OpenAlex.

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  11. [Establishment of rabbit model of benign circumferential esophageal stricture].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2026
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  19. Temperature-modulated separation of therapeutic cells, viral vectors, and exosomes using functional polymers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
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142 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Takeshi OhkiDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Masayuki YamatoInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Masaho OtaDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan.
Ryo TakagiInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Daisuke MurakamiInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan; Graduate School of Science and Engineering, Waseda University, Tokyo, Japan.
Makoto KondoInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan; Graduate School of Science and Engineering, Waseda University, Tokyo, Japan.
Ryo SasakiDepartment of Oral and Maxillofacial Surgery, Tokyo Women's Medical University, Tokyo, Japan.
Hideo NamikiGraduate School of Science and Engineering, Waseda University, Tokyo, Japan.
Teruo OkanoInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan. Electronic address: tokano@abmes.twmu.ac.jp.
Masakazu YamamotoDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan.
Tokyo Women's Medical University · JPWaseda University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND &

aimsThe use of esophageal endoscopic submucosal dissection (ESD) to remove superficial esophageal neoplasms is gradually becoming more common in Japan. However, large-scale esophageal ESD often requires subsequent multiple balloon dilations to prevent postoperative esophageal stricture. We investigated the safety and efficacy of endoscopic transplantation of tissue-engineered autologous oral mucosal epithelial cell sheets in preventing formation of strictures after ESD.

methodsWe performed an open-label, single-arm, single-institute study. We collected specimens of oral mucosal tissue from 9 patients with superficial esophageal neoplasms. Epithelial cell sheets were fabricated ex vivo by culturing isolated cells for 16 days on temperature-responsive cell culture surfaces. After a reduction in temperature, these sheets were endoscopically transplanted directly to the ulcer surfaces of patients who had just undergone ESD. All patients were monitored by endoscopy once a week until epithelialization was complete.

resultsAutologous cell sheets were successfully transplanted to ulcer surfaces using an endoscope. Complete re-epithelialization occurred within a median time of 3.5 weeks. No patients experienced dysphagia, stricture, or other complications following the procedure, except for one patient who had a full circumferential ulceration that expanded to the esophagogastric junction.

conclusionsSutureless, endoscopic transplantation of carrier-free cell sheets composed of autologous oral mucosal epithelial cells safely and effectively promotes re-epithelialization of the esophagus after ESD. Patients in this study did not experience any serious complications. This procedure might be used to prevent stricture formation following ESD and improve patients' quality of life. Further study will be needed to show that stricture formation can be prevented.

Indexed as

Tissue EngineeringAgedAged, 80 and overCells, CulturedDissectionEpithelial CellsEsophageal NeoplasmsEsophageal StenosisEsophagoscopyEsophagusHumansJapanMaleMiddle AgedMouth MucosaTime Factors

Identifiers

PMID22561054
OpenAlexW1965630124

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.