ArticleBMC medical research methodology2012
Quality of DNA extracted from saliva samples collected with the Oragene™ DNA self-collection kit.
Article in BMC medical research methodology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
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- The long-term associations of childhood parental loss with attachment, creativity, and epigenetic regulation.Scientific reports · 2025Article
- Mexican Health and Aging Study Biomarker and Genetic Data Profile.The journals of gerontology. Series A, Biological sciences and medical sciences · 2024Article
- Elucidating a genomic signature associated with behavioral and executive function after moderate to severe pediatric TBI: a systems biology informed approach.Frontiers in systems biology · 2024Article
- Using saliva epigenetic data to develop and validate a multivariable predictor of esophageal cancer status.Epigenomics · 2024Article
- Allele frequency and genotype distribution of the opioid receptor μ-1 (OPRM1) A118G polymorphism in the Western Saudi population.Journal of applied biomedicine · 2023Article
- Isolation of salivary cell-free DNA for cancer detection.PloS one · 2023Article
- Assessment of the Feasibility of a Future Integrated Larger-Scale Epidemiological Study to Evaluate Health Risks of Air Pollution Episodes in Children.International journal of environmental research and public health · 2022Article
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- Salivary Metabolites are Promising Non-Invasive Biomarkers of Hepatocellular Carcinoma and Chronic Liver Disease.Liver cancer international · 2021Article
- Pharmacogenomics at the Point of Care: A Community Pharmacy Project in British Columbia.Journal of personalized medicine · 2020Article
- Contaminating DNA in human saliva alters the detection of variants from whole genome sequencing.Scientific reports · 2020Article
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Authors and funding
10 authors.
Funding
Abstract
backgroundLarge epidemiological studies in DNA biobanks have increasingly used less invasive methods for obtaining DNA samples, such as saliva collection. Although lower amounts of DNA are obtained as compared with blood collection, this method has been widely used because of its more simple logistics and increased response rate. The present study aimed to verify whether a storage time of 8 months decreases the quality of DNA from collected samples.
methodsSaliva samples were collected with an OrageneTM DNA Self-Collection Kit from 4,110 subjects aged 14-15 years. The samples were processed in two aliquots with an 8-month interval between them. Quantitative and qualitative evaluations were carried out in 20% of the samples by spectrophotometry and genotyping. Descriptive analyses and paired t-tests were performed.
resultsThe mean volume of saliva collected was 2.2 mL per subject, yielding on average 184.8 μg DNA per kit. Most samples showed a Ratio of OD differences (RAT) between 1.6 and 1.8 in the qualitative evaluation. The evaluation of DNA quality by TaqMan®, High Resolution Melting (HRM), and restriction fragment length polymorphism-PCR (RFLP-PCR) showed a rate of success of up to 98% of the samples. The sample store time did not reduce either the quantity or quality of DNA extracted with the Oragene kit.
conclusionThe study results showed that a storage period of 8 months at room temperature did not reduce the quality of the DNA obtained. In addition, the use of the Oragene kit during fieldwork in large population-based studies allows for DNA of high quantity and high quality.
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