ArticleTissue engineering. Part C, Methods2012
Decellularized extracellular matrix derived from porcine adipose tissue as a xenogeneic biomaterial for tissue engineering.
Article in Tissue engineering. Part C, Methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Review of Submetatarsal Fat Pad Augmentation for the Treatment and Prevention of Diabetes-Related Foot Ulceration.Journal of foot and ankle research · 2025Pooled it
- Benchmarking of Decellularization Protocols for Small Intestinal Submucosa: Defining the Gold Standard for Functional Tissue Engineering.Materials (Basel, Switzerland) · 2026Article
- Extracellular-Matrix-Based Materials from Decellularized Tissue: Opportunities, Challenges, and Future Directions in Regenerative Medicine.Advanced healthcare materials · 2026Review
- Porcine Bone Extracellular Matrix Hydrogel as a Promising Graft for Bone Regeneration.Gels (Basel, Switzerland) · 2025Article
- Advances and challenges in decellularized adipose tissue based composite hydrogels for adipose tissue regeneration: a review over the last fifteen years.Theranostics · 2025Review
- The Unfulfilled Potential of Synthetic and Biological Hydrogel Membranes in the Treatment of Abdominal Hernias.Gels (Basel, Switzerland) · 2024Review
- Comparison of Stored and Fresh Injectable Acellular Adipose Matrix in Soft Tissue Reconstruction in a Murine Model.Aesthetic plastic surgery · 2024Article
- Effect of Injectable Acellular Adipose Matrix on Soft Tissue Reconstruction in a Murine Model.Aesthetic plastic surgery · 2024Article
- SERS in 3D cell models: a powerful tool in cancer research.Chemical Society reviews · 2024Review
- Biofabrication, biochemical profiling, and in vitro applications of salivary gland decellularized matrices via magnetic bioassembly platforms.Cell and tissue research · 2023Article
- Recent advances in decellularized biomaterials for wound healing.Materials today. Bio · 2023Review
- Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models.Biomolecules · 2022Article
- Decellularization of caprine esophagus using fruit pericarp extract of Sapindus mukorossi.Cell and tissue banking · 2022Article
- Fabrication of Human Skin Equivalents Using Decellularized Extracellular Matrix.Current protocols · 2022Article
- Investigating the Adipogenic Effects of Different Tissue-Derived Decellularized Matrices.Frontiers in bioengineering and biotechnology · 2022Article
- Microstructure and Thermal Property of Designed Alginate-Based Polymeric Composite Foam Materials Containing Biomimetic Decellularized Elastic Cartilage Microscaffolds.Materials (Basel, Switzerland) · 2021Article
- Article
- Bioactivity Comparison of Electrospun PCL Mats and Liver Extracellular Matrix as Scaffolds for HepG2 Cells.Polymers · 2021Article
- Vascular Tissue Engineering: Challenges and Requirements for an Ideal Large Scale Blood Vessel.Frontiers in bioengineering and biotechnology · 2021Review
- The Application of Decellularized Adipose Tissue Promotes Wound Healing.Tissue engineering and regenerative medicine · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells in tissues are surrounded by the extracellular matrix (ECM), a gel-like material of proteins and polysaccharides that are synthesized and secreted by cells. Here we propose that the ECM can be isolated from porcine adipose tissue and holds great promise as a xenogeneic biomaterial for tissue engineering and regenerative medicine. Porcine adipose tissue is easily obtained in large quantities from commonly discarded food waste. Decellularization protocols have been developed for extracting an intact ECM while effectively eliminating xenogeneic epitopes and minimally disrupting the ECM composition. Porcine adipose tissue was defatted by homogenization and centrifugation. It was then decellularized via chemical (1.5 M sodium chloride and 0.5% sodium dodecyl sulfate) and enzymatic treatments (DNase and RNase) with temperature control. After decellularization, immunogenic components such as nucleic acids and α-Gal were significantly reduced. However, abundant ECM components, such as collagen (332.9±12.1 μg/mg ECM dry weight), sulfated glycosaminoglycan (GAG, 85±0.7 μg/mg ECM dry weight), and elastin (152.6±4.5 μg/mg ECM dry weight), were well preserved in the decellularized material. The biochemical and mechanical features of a decellularized ECM supported the adhesion and growth of human cells in vitro. Moreover, the decellularized ECM exhibited biocompatibility, long-term stability, and bioinductivity in vivo. The overall results suggest that the decellularized ECM derived from porcine adipose tissue could be useful as an alternative biomaterial for xenograft tissue engineering.
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