ArticlePlant methods2012
Poinsettia protoplasts - a simple, robust and efficient system for transient gene expression studies.
Article in Plant methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants.Plants (Basel, Switzerland) · 2026Review
- Establishment of protoplast isolation, purification and transient transformation system from Rhododendron petals.Protoplasma · 2026Article
- Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Frontiers in plant science · 2025Article
- An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines.BMC research notes · 2023Article
- Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023Article
- Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu).BMC plant biology · 2023Article
- Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruitFrontiers in plant science · 2023Article
- Uncertainty of EIN2Plant communications · 2020Article
- Optimization of isolation and transfection conditions of maize endosperm protoplasts.Plant methods · 2020Article
- Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration.Plant biotechnology journal · 2018Article
- A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses.Journal of visualized experiments : JoVE · 2018Article
- Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells.PloS one · 2017Article
- Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis.BMC biotechnology · 2016Article
- Characterization of Actin Filament Dynamics during Mitosis in Wheat Protoplasts under UV-B Radiation.Scientific reports · 2016Article
- Post-Translational Modification and Secretion of Azelaic Acid Induced 1 (AZI1), a Hybrid Proline-Rich Protein from Arabidopsis.International journal of molecular sciences · 2016Article
- TheFrontiers in plant science · 2016Article
- Evidence of capsaicin synthase activity of the Pun1-encoded protein and its role as a determinant of capsaicinoid accumulation in pepper.BMC plant biology · 2015Article
- Salt stress in Arabidopsis: lipid transfer protein AZI1 and its control by mitogen-activated protein kinase MPK3.Molecular plant · 2014Article
- Dominant repression by Arabidopsis transcription factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress.International journal of molecular sciences · 2014Article
- Newly developed quantitative transactivation system shows difference in activation by Vitis CBF transcription factors on DRE/CRT elements.Plant methods · 2014Article
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2 authors.
Funding
Abstract
backgroundTransient gene expression systems are indispensable tools in molecular biology. Yet, their routine application is limited to few plant species often requiring substantial equipment and facilities. High chloroplast and chlorophyll content may further impede downstream applications of transformed cells from green plant tissue.
resultsHere, we describe a fast and simple technique for the high-yield isolation and efficient transformation (>70%) of mesophyll-derived protoplasts from red leaves of the perennial plant Poinsettia (Euphorbia pulccherrima). In this method no particular growth facilities or expensive equipments are needed. Poinsettia protoplasts display an astonishing robustness and can be employed in a variety of commonly-used downstream applications, such as subcellular localisation (multi-colour fluorescence) or promoter activity studies. Due to low abundance of chloroplasts or chromoplasts, problems encountered in other mesophyll-derived protoplast systems (particularly autofluorescence) are alleviated. Furthermore, the transgene expression is detectable within 90 minutes of transformation and lasts for several days.
conclusionsThe simplicity of the isolation and transformation procedure renders Poinsettia protoplasts an attractive system for transient gene expression experiments, including multi-colour fluorescence, subcellular localisation and promoter activity studies. In addition, they offer hitherto unknown possibilities for anthocyan research and industrial applications.
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