Evidence map›Paper›PMID 22559319›Full record

ReviewAnnual review of biomedical engineering2012

Optical nanoscopy: from acquisition to analysis.

Travis J Gould, Samuel T Hess, Joerg Bewersdorf

Abstract readReview
In one paragraph

Review in Annual review of biomedical engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Neuro at the Nanoscale: Diffraction-Unlimited Imaging with STED Nanoscopy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2015
    Review
  10. Article
  11. Article
  12. Review
  13. Review
  14. Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Determination of two-photon photoactivation rates of fluorescent proteins.Physical chemistry chemical physics : PCCP · 2013
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Travis J GouldDepartment of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA. travis.gould@yale.edu
Samuel T Hess
Joerg Bewersdorf

Funding

Dynamic and super-resolution imaging of endogenous proteins in Drosophila tissuesRC1GM091791 · NIGMS · YALE UNIVERSITY · PI COOLEY, LYNN · 2009 to 2010
$947k
The Role of Rafts in Virus-Induced Membrane RemodelingK25AI065459 · NIAID · UNIVERSITY OF MAINE ORONO · PI HESS, SAMUEL T · 2005 to 2009
$663k
The Role of the Actin Cytoskeleton and Cholesterol in Influenza Virus AssemblyR15GM094713 · NIGMS · UNIVERSITY OF MAINE ORONO · PI HESS, SAMUEL T · 2010 to 2010
$425k
Realization of TIRF-STED microscopy and its application to fast nanoscale imagingF32GM096859 · NIGMS · YALE UNIVERSITY · PI GOULD, TRAVIS JOHN · 2011 to 2012
$101k
NIAID NIH HHS K25 AI065459NIAID NIH HHS K25AI65459NIGMS NIH HHS F32 GM096859NIGMS NIH HHS F32GM096859NIGMS NIH HHS R15 GM094713NIGMS NIH HHS R15GM094713NIGMS NIH HHS RC1 GM091791
6 · The paper itself

Abstract

Recent advances in far-field microscopy have demonstrated that fluorescence imaging is possible at resolutions well below the long-standing diffraction limit. By exploiting photophysical properties of fluorescent probe molecules, this new class of methods yields a resolving power that is fundamentally diffraction unlimited. Although these methods are becoming more widely used in biological imaging, they must be complemented by suitable data analysis approaches if their potential is to be fully realized. Here we review the basic principles of diffraction-unlimited microscopy and how these principles influence the selection of available algorithms for data analysis. Furthermore, we provide an overview of existing analysis strategies and discuss their application.

Indexed as

AlgorithmsBiomedical EngineeringFluorescent DyesHumansImage Processing, Computer-AssistedImaging, Three-DimensionalMicroscopyMicroscopy, FluorescenceModels, StatisticalNanotechnologyOptics and PhotonicsFluorescent Dyes

Identifiers

PMID22559319
PMCPMC3568761

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.