ArticleBMC biotechnology2012
Untranslated regions of diverse plant viral RNAs vary greatly in translation enhancement efficiency.
Article in BMC biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- From Structure to Function of Promoters and 5'UTRs in Maize.International journal of molecular sciences · 2026Review
- RNA elements and their biotechnological applications in plants.The New phytologist · 2025Review
- Advances in Understanding the Mechanism of Cap-Independent Cucurbit Aphid-Borne Yellows Virus Protein Synthesis.International journal of molecular sciences · 2023Article
- Transcriptional and post-transcriptional controls for tuning gene expression in plants.Current opinion in plant biology · 2023Review
- Maximizing the Production of Recombinant Proteins in Plants: From Transcription to Protein Stability.International journal of molecular sciences · 2022Review
- Design and Analysis of Native Photorespiration Gene Motifs of Promoter Untranslated Region Combinations Under Short Term Abiotic Stress Conditions.Frontiers in plant science · 2022Article
- Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology.Frontiers in molecular biosciences · 2021Review
- Development of a Virus-Based Reporter System for Functional Analysis of Plant rRNA Gene Promoter.Frontiers in microbiology · 2021Article
- Robot Cookies - Plant Cell Packs as an Automated High-Throughput Screening Platform Based on Transient Expression.Frontiers in bioengineering and biotechnology · 2020Article
- The 3' Untranslated Region of a Plant Viral RNA Directs Efficient Cap-Independent Translation in Plant and Mammalian Systems.Pathogens (Basel, Switzerland) · 2019Article
- Improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions.Plant methods · 2019Article
- Chimeric 3' flanking regions strongly enhance gene expression in plants.Plant biotechnology journal · 2018Article
- A Dual Interaction Between the 5'- and 3'-Ends of the Melon Necrotic Spot Virus (MNSV) RNA Genome Is Required for Efficient Cap-Independent Translation.Frontiers in plant science · 2018Article
- Non-canonical Translation in Plant RNA Viruses.Frontiers in plant science · 2017Review
- Noncoding RNAs of Plant Viruses and Viroids: Sponges of Host Translation and RNA Interference Machinery.Molecular plant-microbe interactions : MPMI · 2016Review
- 5' and 3' Untranslated Regions Strongly Enhance Performance of Geminiviral Replicons in Nicotiana benthamiana Leaves.Frontiers in plant science · 2016Article
- Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system.BMC biotechnology · 2014Article
- Functional expression of human α9* nicotinic acetylcholine receptors in X. laevis oocytes is dependent on the α9 subunit 5' UTR.PloS one · 2013Article
- A 28 nt long synthetic 5'UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants.BMC biotechnology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWhole plants or plant cell cultures can serve as low cost bioreactors to produce massive amounts of a specific protein for pharmacological or industrial use. To maximize protein expression, translation of mRNA must be optimized. Many plant viral RNAs harbor extremely efficient translation enhancers. However, few of these different translation elements have been compared side-by-side. Thus, it is unclear which are the most efficient translation enhancers. Here, we compare the effects of untranslated regions (UTRs) containing translation elements from six plant viruses on translation in wheat germ extract and in monocotyledenous and dicotyledenous plant cells.
resultsThe highest expressing uncapped mRNAs contained viral UTRs harboring Barley yellow dwarf virus (BYDV)-like cap-independent translation elements (BTEs). The BYDV BTE conferred the most efficient translation of a luciferase reporter in wheat germ extract and oat protoplasts, while uncapped mRNA containing the BTE from Tobacco necrosis virus-D translated most efficiently in tobacco cells. Capped mRNA containing the Tobacco mosaic virus omega sequence was the most efficient mRNA in tobacco cells. UTRs from Satellite tobacco necrosis virus, Tomato bushy stunt virus, and Crucifer-infecting tobamovirus (crTMV) did not stimulate translation efficiently. mRNA with the crTMV 5' UTR was unstable in tobacco protoplasts.
conclusionsBTEs confer the highest levels of translation of uncapped mRNAs in vitro and in vivo, while the capped omega sequence is most efficient in tobacco cells. These results provide a basis for understanding mechanisms of translation enhancement, and for maximizing protein synthesis in cell-free systems, transgenic plants, or in viral expression vectors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.