ArticleBMC biotechnology2012
Solution-based targeted genomic enrichment for precious DNA samples.
Article in BMC biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Automated Purification of DNA Origami with SPRI Beads.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- The minimal amount of starting DNA for Agilent's hybrid capture-based targeted massively parallel sequencing.Scientific reports · 2016Article
- Comprehensive genetic testing with ethnic-specific filtering by allele frequency in a Japanese hearing-loss population.Clinical genetics · 2016Article
- Active promoters give rise to false positive 'Phantom Peaks' in ChIP-seq experiments.Nucleic acids research · 2015Article
- Mutations in LOXHD1 gene cause various types and severities of hearing loss.The Annals of otology, rhinology, and laryngology · 2015Article
- De novo mutation in X-linked hearing loss-associated POU3F4 in a sporadic case of congenital hearing loss.The Annals of otology, rhinology, and laryngology · 2015Article
- USH2 caused by GPR98 mutation diagnosed by massively parallel sequencing in advance of the occurrence of visual symptoms.The Annals of otology, rhinology, and laryngology · 2015Article
- Hearing loss caused by a P2RX2 mutation identified in a MELAS family with a coexisting mitochondrial 3243AG mutation.The Annals of otology, rhinology, and laryngology · 2015Article
- Novel PTPRQ mutations identified in three congenital hearing loss patients with various types of hearing loss.The Annals of otology, rhinology, and laryngology · 2015Article
- Article
- Comprehensive genetic analysis of complement and coagulation genes in atypical hemolytic uremic syndrome.Journal of the American Society of Nephrology : JASN · 2014Article
- Advancing genetic testing for deafness with genomic technology.Journal of medical genetics · 2013Article
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Authors and funding
3 authors.
Funding
Abstract
backgroundSolution-based targeted genomic enrichment (TGE) protocols permit selective sequencing of genomic regions of interest on a massively parallel scale. These protocols could be improved by: 1) modifying or eliminating time consuming steps; 2) increasing yield to reduce input DNA and excessive PCR cycling; and 3) enhancing reproducible.
resultsWe developed a solution-based TGE method for downstream Illumina sequencing in a non-automated workflow, adding standard Illumina barcode indexes during the post-hybridization amplification to allow for sample pooling prior to sequencing. The method utilizes Agilent SureSelect baits, primers and hybridization reagents for the capture, off-the-shelf reagents for the library preparation steps, and adaptor oligonucleotides for Illumina paired-end sequencing purchased directly from an oligonucleotide manufacturing company.
conclusionsThis solution-based TGE method for Illumina sequencing is optimized for small- or medium-sized laboratories and addresses the weaknesses of standard protocols by reducing the amount of input DNA required, increasing capture yield, optimizing efficiency, and improving reproducibility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.