ArticlePloS one2012
Invasion of Wolbachia into Anopheles and other insect germlines in an ex vivo organ culture system.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Control of arboviruses vectors using biological control by Wolbachia pipientis: a short review.Archives of microbiology · 2022Pooled it
- Primary transcript enrichment reveals a density-dependentFrontiers in microbiology · 2026Article
- A Light in the Dark: Uncovering Wolbachia-Host Interactions Using Fluorescence Imaging.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Sex determination systems as the interface between male-killing bacteria and their hosts.Proceedings. Biological sciences · 2022Review
- Stable high-density and maternally inherited Wolbachia infections in Anopheles moucheti and Anopheles demeilloni mosquitoes.Current biology : CB · 2021Article
- The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions.Frontiers in microbiology · 2021Review
- Review
- Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus.Journal of visualized experiments : JoVE · 2017Article
- Dietary saccharides and sweet tastants have differential effects on colonization ofBiology open · 2017Article
- Mechanisms of Horizontal Cell-to-Cell Transfer of Wolbachia spp. in Drosophila melanogaster.Applied and environmental microbiology · 2017Article
- The rich somatic life of Wolbachia.MicrobiologyOpen · 2016Review
- The microbiome modulates arbovirus transmission in mosquitoes.Current opinion in virology · 2015Review
- Mutual exclusion of Asaia and Wolbachia in the reproductive organs of mosquito vectors.Parasites & vectors · 2015Article
- Native microbiome impedes vertical transmission of Wolbachia in Anopheles mosquitoes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Metagenomics, paratransgenesis and the Anopheles microbiome: a portrait of the geographical distribution of the anopheline microbiota based on a meta-analysis of reported taxa.Memorias do Instituto Oswaldo Cruz · 2014Review
- Wolbachia enhances West Nile virus (WNV) infection in the mosquito Culex tarsalis.PLoS neglected tropical diseases · 2014Article
- Transinfection: a method to investigate Wolbachia-host interactions and control arthropod-borne disease.Insect molecular biology · 2014Review
- Temperature alters Plasmodium blocking by Wolbachia.Scientific reports · 2014Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The common bacterial endosymbiont Wolbachia manipulates its host's reproduction to promote its own maternal transmission, and can interfere with pathogen development in many insects making it an attractive agent for the control of arthropod-borne disease. However, many important species, including Anopheles mosquitoes, are uninfected. Wolbachia can be artificially transferred between insects in the laboratory but this can be a laborious and sometimes fruitless process. We used a simple ex vivo culturing technique to assess the suitability of Wolbachia-host germline associations. Wolbachia infects the dissected germline tissue of multiple insect species when the host tissue and bacteria are cultured together. Ovary and testis infection occurs in a density-dependent manner. Wolbachia strains are more capable of invading the germline of their native or closely related rather than divergent hosts. The ability of Wolbachia to associate with the germline of novel hosts is crucial for the development of stably-transinfected insect lines. Rapid assessment of the suitability of a strain-host combination prior to transinfection may dictate use of a particular Wolbachia strain. Furthermore, the cultured germline tissues of two major Anopheline vectors of Plasmodium parasites are susceptible to Wolbachia infection. This finding further enhances the prospect of using Wolbachia for the biological control of malaria.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.