ArticlePloS one2012
Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Circadian Behavioral and Bioluminescence Rhythms in theJournal of biological rhythms · 2026Article
- Neuroendocrine effects of the duper mutation in Syrian hamsters: a role forFrontiers in physiology · 2024Article
- Intense light-elicited alveolar type 2-specific circadian PER2 protects from bacterial lung injury via BPIFB1.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model.Theranostics · 2022Article
- Anatomical Methods to Study the Suprachiasmatic Nucleus.Methods in molecular biology (Clifton, N.J.) · 2022Article
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- A Role for the Adenosine ADORA2B Receptor in Midazolam Induced Cognitive Dysfunction.Current pharmaceutical design · 2020Article
- Removing Short Wavelengths From Polychromatic White Light Attenuates Circadian Phase Resetting in Rats.Frontiers in neuroscience · 2019Article
- Suprachiasmatic function in a circadian period mutant: Duper alters light-induced activation of vasoactive intestinal peptide cells and PERIOD1 immunostaining.The European journal of neuroscience · 2018Article
- The Period 2 Enhancer Nobiletin as Novel Therapy in Murine Models of Circadian Disruption Resembling Delirium.Critical care medicine · 2018Article
- Functionally Complete Excision of Conditional Alleles in the Mouse Suprachiasmatic Nucleus by Vgat-ires-Cre.Journal of biological rhythms · 2018Article
- Article
- Differential localization of PER1 and PER2 in the brain master circadian clock.The European journal of neuroscience · 2017Article
- Cell type-specific transcriptomics of hypothalamic energy-sensing neuron responses to weight-loss.eLife · 2015Article
- Clock genes control cortical critical period timing.Neuron · 2015Article
- Dim light at night disrupts molecular circadian rhythms and increases body weight.Journal of biological rhythms · 2013Article
- Sleep disorders and depression: brief review of the literature, case report, and nonpharmacologic interventions for depression.Clinical interventions in aging · 2013Review
- Heterogeneous expression of the core circadian clock proteins among neuronal cell types in mouse retina.PloS one · 2012Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Research on the mechanisms underlying circadian rhythmicity and the response of brain and body clocks to environmental and physiological challenges requires assessing levels of circadian clock proteins. Too often, however, it is difficult to acquire antibodies that specifically and reliably label these proteins. Many of these antibodies also lack appropriate validation. The goal of this project was to generate and characterize antibodies against several circadian clock proteins. We examined mice and hamsters at peak and trough times of clock protein expression in the suprachiasmatic nucleus (SCN). In addition, we confirmed specificity by testing the antibodies on mice with targeted disruption of the relevant genes. Our results identify antibodies against PER1, PER2, BMAL1 and CLOCK that are useful for assessing circadian clock proteins in the SCN by immunocytochemistry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.