ArticlePloS one2012
Deletion of Dicer in smooth muscle affects voiding pattern and reduces detrusor contractility and neuroeffector transmission.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Mir-29 repression in bladder outlet obstruction contributes to matrix remodeling and altered stiffness.PloS one · 2013Trial
- Lack of expression of miR-29a/b1 impairs bladder function in male mice.Disease models & mechanisms · 2023Article
- NG2/CSPG4, CD146/MCAM and VAP1/AOC3 are regulated by myocardin-related transcription factors in smooth muscle cells.Scientific reports · 2021Article
- MicroRNA-dependent regulation of KLF4 by glucose in vascular smooth muscle.Journal of cellular physiology · 2018Article
- Concordant miRNA and mRNA expression profiles in humans and mice with bladder outlet obstruction.American journal of clinical and experimental urology · 2018Article
- Elevated Glucose Levels Promote Contractile and Cytoskeletal Gene Expression in Vascular Smooth Muscle via Rho/Protein Kinase C and Actin Polymerization.The Journal of biological chemistry · 2016Article
- Epithelium-Intrinsic MicroRNAs Contribute to Mucosal Immune Homeostasis by Promoting M-Cell Maturation.PloS one · 2016Article
- MicroRNA MiR-199a-5p regulates smooth muscle cell proliferation and morphology by targeting WNT2 signaling pathway.The Journal of biological chemistry · 2015Article
- Simulated microgravity inhibits L-type calcium channel currents partially by the up-regulation of miR-103 in MC3T3-E1 osteoblasts.Scientific reports · 2015Article
- Article
- Detrusor induction of miR-132/212 following bladder outlet obstruction: association with MeCP2 repression and cell viability.PloS one · 2015Article
- Myocardin Family Members Drive Formation of Caveolae.PloS one · 2015Article
- Deciphering microRNA code in pain and inflammation: lessons from bladder pain syndrome.Cellular and molecular life sciences : CMLS · 2013Review
- A panel of 4 microRNAs facilitates the prediction of left ventricular contractility after acute myocardial infarction.PloS one · 2013Article
- Regulation of vascular smooth muscle mechanotransduction by microRNAs and L-type calcium channels.Communicative & integrative biology · 2013Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs have emerged as important regulators of smooth muscle phenotype and may play important roles in pathogenesis of various smooth muscle related disease states. The aim of this study was to investigate the role of miRNAs for urinary bladder function. We used an inducible and smooth muscle specific Dicer knockout (KO) mouse which resulted in significantly reduced levels of miRNAs, including miR-145, miR-143, miR-22, miR125b-5p and miR-27a, from detrusor preparations without mucosa. Deletion of Dicer resulted in a disturbed micturition pattern in vivo and reduced depolarization-induced pressure development in the isolated detrusor. Furthermore, electrical field stimulation revealed a decreased cholinergic but maintained purinergic component of neurogenic activation in Dicer KO bladder strips. The ultrastructure of detrusor smooth muscle cells was well maintained, and the density of nerve terminals was similar. Western blotting demonstrated reduced contents of calponin and desmin. Smooth muscle α-actin, SM22α and myocardin were unchanged. Activation of strips with exogenous agonists showed that depolarization-induced contraction was preferentially reduced; ATP- and calyculin A-induced contractions were unchanged. Quantitative real time PCR and western blotting demonstrated reduced expression of Cav1.2 (Cacna1c). It is concluded that smooth muscle miRNAs play an important role for detrusor contractility and voiding pattern of unrestrained mice. This is mediated in part via effects on expression of smooth muscle differentiation markers and L-type Ca(2+) channels in the detrusor.
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