ArticlePloS one2012
Experimental verification of a predicted intronic microRNA in human NGFR gene with a potential pro-apoptotic function.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
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- Development of an immune-related gene prognostic risk model and identification of an immune infiltration signature in the tumor microenvironment of colon cancer.BMC gastroenterology · 2023Article
- Identification of Prognostic Genes in Gliomas Based on Increased Microenvironment Stiffness.Cancers · 2022Article
- A Novel miRNA Located in theBioMed research international · 2022Article
- Implication of TrkC-miR2 in neurotrophin signalling pathway regulation through NGFR transcript targeting.Journal of cellular and molecular medicine · 2021Article
- Some Molecular and Cellular Stress Mechanisms Associated with Neurodegenerative Diseases and Atherosclerosis.International journal of molecular sciences · 2021Review
- Screening of differentially expressed miRNAs during osteogenic/odontogenic differentiation of human dental pulp stem cells exposed to mechanical stress.American journal of translational research · 2021Article
- MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4.OncoTargets and therapy · 2020Article
- Screening and identification of potential novel biomarker for diagnosis of complicated Plasmodium vivax malaria.Journal of translational medicine · 2018Article
- Identification of a novel intergenic miRNA located between the human DDC and COBL genes with a potential function in cell cycle arrest.Molecular and cellular biochemistry · 2018Article
- Functional intron-derived miRNAs and host-gene expression in plants.Plant methods · 2018Article
- Expression and Function of hsa-miR-6165 in Human Cell Lines and During the NT2 Cell Neural Differentiation Process.Journal of molecular neuroscience : MN · 2017Article
- A novel microRNA located in theThe Journal of biological chemistry · 2017Article
- Alternative splicing of the OCC-1 gene generates three splice variants and a novel exonic microRNA, which regulate the Wnt signaling pathway.RNA (New York, N.Y.) · 2017Article
- Experimental verification of a predicted novel microRNA located in human PIK3CA gene with a potential oncogenic function in colorectal cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Molecular Changes in the Nasal Cavity after N, N-dimethyl-p-toluidine Exposure.Toxicologic pathology · 2016Article
- Hsa-miR-590-5p Interaction with SMAD3 Transcript Supports Its Regulatory Effect on The TGFβ Signaling Pathway.Cell journal · 2016Article
- Experimental verification of a conserved intronic microRNA located in the human TrkC gene with a cell type-dependent apoptotic function.Cellular and molecular life sciences : CMLS · 2015Article
- Regulatory networks between neurotrophins and miRNAs in brain diseases and cancers.Acta pharmacologica Sinica · 2015Review
- Human RNAi pathway: crosstalk with organelles and cells.Functional & integrative genomics · 2014Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurotrophins (NTs) are a family of secreted growth factor proteins primarily involved in the regulation of survival and appropriate development of neural cells, functioning by binding to their specific (TrkA, TtkB, and TrkC) and/or common NGFR receptor. NGFR is the common receptor of NTs, binding with low-affinity to all members of the family. Among different functions assigned to NGFR, it is also involved in apoptosis induction and tumorigenesis processes. Interestingly, some of the functions of NGFR appear to be ligand-independent, suggesting a probable involvement of non-coding RNA residing within the sequence of the gene. Here, we are reporting the existence of a conserved putative microRNA, named Hsa-mir-6165 [EBI accession#: FR873488]. Transfection of a DNA segment corresponding to the pre-mir-6165 sequence in Hela cell line caused the generation of mature exogenous mir-6165 (a ∼200,000 fold overexpression). Furthermore, using specific primers, we succeeded to detect the endogenous expression of mir-6165 in several glioma cell lines and glioma primary tumors known to express NGFR. Similar to the pro-apoptotic role of NGFR in some cell types, overexpression of pre-mir-6165 in U87 cell line resulted in an elevated rate of apoptosis. Moreover, coordinated with the increased level of mir-6165 in the transfected U87 cell line, two of its predicted target genes (Pkd1 and DAGLA) were significantly down-regulated. The latter findings suggest that some of the previously attributed functions of NGFR could be explained indirectly by co-transcription of mir-6165 in the cells.
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