ArticleGMS Krankenhaushygiene interdisziplinar2012
XTT assay of ex vivo saliva biofilms to test antimicrobial influences.
Article in GMS Krankenhaushygiene interdisziplinar, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
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- SynergisticFrontiers in cellular and infection microbiology · 2024Article
- Evaluating models and assessment techniques for understanding oral biofilm complexity.MicrobiologyOpen · 2023Review
- Alternative Antibiotics in Dentistry: Antimicrobial Peptides.Pharmaceutics · 2022Review
- Antimicrobial Effects of Gum Arabic-Silver Nanoparticles against Oral Pathogens.Bioinorganic chemistry and applications · 2022Article
- Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion.Nanomaterials (Basel, Switzerland) · 2021Article
- A New Insight into the Bactericidal Mechanism of 405 nm Blue Light-Emitting-Diode against Dairy SourcedFoods (Basel, Switzerland) · 2021Article
- Effects of lipid emulsions on the formation of Escherichia coli-Candida albicans mixed-species biofilms on PVC.Scientific reports · 2021Article
- Influence of Nutrient Media Compared to Human Synovial Fluid on the Antibiotic Susceptibility and Biofilm Gene Expression of Coagulase-NegativeAntibiotics (Basel, Switzerland) · 2021Article
- Effect of Silver Diamine Fluoride on Reducing Candida albicans Adhesion on Dentine.Mycopathologia · 2020Article
- Automation of antimicrobial activity screening.AMB Express · 2016Article
- Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis.BioMed research international · 2016Article
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveMany dental diseases are attributable to biofilms. The screening of antimicrobial substances, in particular, requires a high sample throughput and a realistic model, the evaluation must be as quick and as simple as possible. For this purpose, a colorimetric assay of the tetrazolium salt XTT (sodium 3'-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) converted by saliva biofilms is recommended. Cleavage of XTT by dehydrogenase enzymes of metabolically active cells in biofilms yields a highly colored formazan product which is measured photometrically. MATERIALS AND
methodThe suitability of the XTT assay for detecting the vitality of ex vivo saliva biofilms was tested to determine the efficacy of chlorhexidine and ozone versus saliva biofilms grown on titanium discs.
resultsThe XTT method lends itself to testing the vitality of microorganisms in saliva biofilms. The sensitivity of the arrays requires a specific minimum number of pathogens, this number being different for planktonic bacteria and those occurring in biofilms. The antibacterial effect after treatment with chlorhexidine or ozone was measured by XTT conversion that was significantly reduced. The antimicrobial efficacy of 60 s 0.5% and 0.1% chlorhexidine treatment was equal and comparable with 60 s ozone treatment.
conclusionThe XTT assay is a suitable method to determine the vitality in saliva biofilms, permitting assessment of the efficacy of antimicrobial substances. Its quick and easy applicability renders it especially suitable for screening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.