ArticleFrontiers in microbiology2012
Mutational Analyses of the Enzymes Involved in the Metabolism of Hydrogen by the Hyperthermophilic Archaeon Pyrococcus furiosus.
Article in Frontiers in microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
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- Revolutionizing bio-hydrogen production: smart integration of nanotechnology, microbial engineering, and circular waste valorisation.Bioresources and bioprocessing · 2026Review
- Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism.Frontiers in microbiology · 2025Article
- Manipulating Fermentation Pathways in the Hyperthermophilic ArchaeonApplied and environmental microbiology · 2023Article
- Optimizing Strategies for Bio-Based Ethanol Production Using Genome-Scale Metabolic Modeling of the Hyperthermophilic Archaeon, Pyrococcus furiosus.Applied and environmental microbiology · 2023Article
- Hydrogenase and Nitrogenase: Key Catalysts in Biohydrogen Production.Molecules (Basel, Switzerland) · 2023Review
- Formate and hydrogen in hydrothermal vents and their use by extremely thermophilic methanogens and heterotrophs.Frontiers in microbiology · 2023Review
- Proteome-wide 3D structure prediction provides insights into the ancestral metabolism of ancient archaea and bacteria.Nature communications · 2022Article
- Conserved and lineage-specific hypothetical proteins may have played a central role in the rise and diversification of major archaeal groups.BMC biology · 2022Article
- Active lithoautotrophic and methane-oxidizing microbial community in an anoxic, sub-zero, and hypersaline High Arctic spring.The ISME journal · 2022Article
- Genome-Scale Metabolic Model ofmSystems · 2021Article
- The Piezo-Hyperthermophilic ArchaeonFrontiers in microbiology · 2021Article
- pH Homeostasis and Sodium Ion Pumping by Multiple Resistance and pH Antiporters inFrontiers in microbiology · 2021Article
- Molecular Hydrogen, a Neglected Key Driver of Soil Biogeochemical Processes.Applied and environmental microbiology · 2019Review
- Characterization of membrane-bound sulfane reductase: A missing link in the evolution of modern day respiratory complexes.The Journal of biological chemistry · 2018Article
- Biotechnology of extremely thermophilic archaea.FEMS microbiology reviews · 2018Review
- Two functionally distinct NADPThe Journal of biological chemistry · 2017Article
- Radiation resistance in thermophiles: mechanisms and applications.World journal of microbiology & biotechnology · 2017Review
- Genetic analyses of the functions of [NiFe]-hydrogenase maturation endopeptidases in the hyperthermophilic archaeon Thermococcus kodakarensis.Extremophiles : life under extreme conditions · 2017Article
- High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii.Scientific reports · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrococcus furiosus grows optimally near 100°C by fermenting carbohydrates to produce hydrogen (H(2)) or, if elemental sulfur (S(0)) is present, hydrogen sulfide instead. It contains two cytoplasmic hydrogenases, SHI and SHII, that use NADP(H) as an electron carrier and a membrane-bound hydrogenase (MBH) that utilizes the redox protein ferredoxin. We previously constructed deletion strains lacking SHI and/or SHII and showed that they exhibited no obvious phenotype. This study has now been extended to include biochemical analyses and growth studies using the ΔSHI and ΔSHII deletion strains together with strains lacking a functional MBH (ΔmbhL). Hydrogenase activity in cytoplasmic extracts of various strains demonstrate that SHI is responsible for most of the cytoplasmic hydrogenase activity. The ΔmbhL strain showed no growth in the absence of S(0), confirming the hypothesis that, in the absence of S(0), MBH is the only enzyme that can dispose of reductant (in the form of H(2)) generated during sugar oxidation. Under conditions of limiting sulfur, a small but significant amount of H(2) was produced by the ΔmbhL strain, showing that SHI can produce H(2) from NADPH in vivo, although this does not enable growth of ΔmbhL in the absence of S(0). We propose that the physiological function of SHI is to recycle H(2) and provide a link between external H(2) and the intracellular pool of NADPH needed for biosynthesis. This likely has a distinct energetic advantage in the environment, but it is clearly not required for growth of the organism under the usual laboratory conditions. The function of SHII, however, remains unknown.
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