ArticleThe Journal of biological chemistry2012
N- and C-terminal transactivation domains of GATA1 protein coordinate hematopoietic program.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Review
- Enhancement of PRMT6 binding to a novel germlineHaematologica · 2024Article
- The abundance of the short GATA1 isoform affects megakaryocyte differentiation and leukemic predisposition in mice.Experimental hematology & oncology · 2024Article
- GATA1 in Normal and Pathologic Megakaryopoiesis and Platelet Development.Advances in experimental medicine and biology · 2024Review
- The novel GATA1-interacting protein HES6 is an essential transcriptional cofactor for human erythropoiesis.Nucleic acids research · 2023Article
- Heterozygous variants in GATA2 contribute to DCML deficiency in mice by disrupting tandem protein binding.Communications biology · 2022Article
- Exploring the Leukemogenic Potential of GATA-1Antioxidants (Basel, Switzerland) · 2021Article
- FAM122A Inhibits Erythroid Differentiation through GATA1.Stem cell reports · 2020Article
- Mitochondria Biogenesis Modulates Iron-Sulfur Cluster Synthesis to Increase Cellular Iron Uptake.DNA and cell biology · 2020Article
- Evolutionary Dynamics of the SKN-1 → MED → END-1,3 Regulatory Gene Cascade inG3 (Bethesda, Md.) · 2020Article
- GATA1 mutations in red cell disorders.IUBMB life · 2020Review
- GATA-1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562.Journal of cellular physiology · 2019Article
- Hematopoietic Differentiation of Human Pluripotent Stem Cells: HOX and GATA Transcription Factors as Master Regulators.Current genomics · 2019Review
- CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome.Molecular therapy. Nucleic acids · 2017Article
- GATA1 Binding Kinetics on Conformation-Specific Binding Sites Elicit Differential Transcriptional Regulation.Molecular and cellular biology · 2016Article
- GATA1 Activity Governed by Configurations ofFrontiers in oncology · 2016Review
- Down syndrome and leukemia: insights into leukemogenesis and translational targets.Translational pediatrics · 2015Review
- GATA transcription factors in vertebrates: evolutionary, structural and functional interplay.Molecular genetics and genomics : MGG · 2014Article
- Contribution of GATA1 dysfunction to multi-step leukemogenesis.Cancer science · 2012Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factor GATA1 regulates the expression of a cluster of genes important for hematopoietic cell differentiation toward erythroid and megakaryocytic lineages. Three functional domains have been identified in GATA1, a transactivation domain located in the N terminus (N-TAD) and two zinc finger domains located in the middle of the molecule. Although N-TAD is known as a solitary transactivation domain for GATA1, clinical observations in Down syndrome leukemia suggest that there may be additional transactivation domains. In this study, we found in reporter co-transfection assays that transactivation activity of GATA1 was markedly reduced by deletion of the C-terminal 95 amino acids without significant attenuation of the DNA binding activity or self-association potential. We therefore generated transgenic mouse lines that expressed GATA1 lacking the C-terminal region (GATA1-ΔCT). When we crossed these transgenic mouse lines to the Gata1-deficient mouse, we found that the GATA1-ΔCT transgene rescued Gata1-deficient mice from embryonic lethality. The embryos rescued with an almost similar level of GATA1-ΔCT to endogenous GATA1 developed beyond embryonic 13.5 days, showing severe anemia with accumulation of immature erythroid cells, as was the case for the embryos rescued by endogenous levels of GATA1 lacking N-TAD (GATA1-ΔNT). Distinct sets of target genes were affected in the embryos rescued by GATA1-ΔCT and GATA1-ΔNT. We also found attenuated GATA1 function in cell cycle control of immature megakaryocytes in both lines of rescued embryos. These results thus demonstrate that GATA1 has two independent transactivation domains, N-TAD and C-TAD. Both N-TAD and C-TAD retain redundant as well as specific activities for proper hematopoiesis in vivo.
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