ArticleThe Journal of biological chemistry2012
The contribution of RCK domains to human BK channel allosteric activation.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Molecular determinants of cholesterol binding to BK channel-forming slo1 subunits.Archives of biochemistry and biophysics · 2026Article
- Two-step voltage-sensor activation of the human KNature communications · 2026Article
- Structural basis of voltage-dependent gating in BK channels.Nature communications · 2025Article
- Mapping the contribution of the C-linker domain to gating polarity in CNBD channels.Biophysical journal · 2024Article
- Fifty years of gating currents and channel gating.The Journal of general physiology · 2023Review
- Inner pore hydration free energy controls the activation of big potassium channels.Biophysical journal · 2023Article
- An allosteric modulator activates BK channels by perturbing coupling between CaNature communications · 2022Article
- Regulatory mechanisms of mitochondrial BKChannels (Austin, Tex.) · 2021Review
- BK Channel Gating Mechanisms: Progresses Toward a Better Understanding of Variants Linked Neurological Diseases.Frontiers in physiology · 2021Review
- Coupling of CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- BK ablation attenuates osteoblast bone formation via integrin pathway.Cell death & disease · 2019Article
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- Physiological Roles and Therapeutic Potential of CaFrontiers in molecular neuroscience · 2018Review
- Threading the biophysics of mammalian Slo1 channels onto structures of an invertebrate Slo1 channel.The Journal of general physiology · 2017Review
- Large-conductance CaThe Journal of physiology · 2017Article
- Understanding the conformational motions of RCK gating rings.The Journal of general physiology · 2017Review
- Structural basis for gating the high-conductance CaNature · 2017Article
- Extrapolating microdomain Ca(2+) dynamics using BK channels as a Ca(2+) sensor.Scientific reports · 2016Article
- Putative calcium-binding domains of the Caenorhabditis elegans BK channel are dispensable for intoxication and ethanol activation.Genes, brain, and behavior · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Large conductance voltage- and Ca(2+)-activated K(+) (BK) channels are potent regulators of cellular processes including neuronal firing, synaptic transmission, cochlear hair cell tuning, insulin release, and smooth muscle tone. Their unique activation pathway relies on structurally distinct regulatory domains including one transmembrane voltage-sensing domain (VSD) and two intracellular high affinity Ca(2+)-sensing sites per subunit (located in the RCK1 and RCK2 domains). Four pairs of RCK1 and RCK2 domains form a Ca(2+)-sensing apparatus known as the "gating ring." The allosteric interplay between voltage- and Ca(2+)-sensing apparati is a fundamental mechanism of BK channel function. Using voltage-clamp fluorometry and UV photolysis of intracellular caged Ca(2+), we optically resolved VSD activation prompted by Ca(2+) binding to the gating ring. The sudden increase of intracellular Ca(2+) concentration ([Ca(2+)](i)) induced a hyperpolarizing shift in the voltage dependence of both channel opening and VSD activation, reported by a fluorophore labeling position 202, located in the upper side of the S4 transmembrane segment. The neutralization of the Ca(2+) sensor located in the RCK2 domain abolished the effect of [Ca(2+)](i) increase on the VSD rearrangements. On the other hand, the mutation of RCK1 residues involved in Ca(2+) sensing did not prevent the effect of Ca(2+) release on the VSD, revealing a functionally distinct interaction between RCK1 and RCK2 and the VSD. A statistical-mechanical model quantifies the complex thermodynamics interplay between Ca(2+) association in two distinct sites, voltage sensor activation, and BK channel opening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.