ArticlePlant physiology2012
Looking deep inside: detection of low-abundance proteins in leaf extracts of Arabidopsis and phloem exudates of pumpkin.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Toward simple, rapid, and deep plant proteome analysis with an in-cell proteomics strategy.bioRxiv : the preprint server for biology · 2025Article
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- Unraveling the Roles of Vascular Proteins Using Proteomics.Molecules (Basel, Switzerland) · 2021Review
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- ArabidopsisPlant physiology · 2018Article
- An Aphid Effector Targets Trafficking Protein VPS52 in a Host-Specific Manner to Promote Virulence.Plant physiology · 2017Article
- Vascular Sap Proteomics: Providing Insight into Long-Distance Signaling during Stress.Frontiers in plant science · 2016Review
- Expect the Unexpected Enrichment of "Hidden Proteome" of Seeds and Tubers by Depletion of Storage Proteins.Frontiers in plant science · 2016Article
- Advances in plant proteomics toward improvement of crop productivity and stress resistancex.Frontiers in plant science · 2015Review
- An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of Arabidopsis protein complexes.Nature protocols · 2015Article
- Computational prediction of candidate proteins for S-nitrosylation in Arabidopsis thaliana.PloS one · 2014Article
- Plant proteomics in India and Nepal: current status and challenges ahead.Physiology and molecular biology of plants : an international journal of functional plant biology · 2013Review
- Plant cell wall proteomics: the leadership of Arabidopsis thaliana.Frontiers in plant science · 2013Article
- Functional proteomics of barley and barley chloroplasts - strategies, methods and perspectives.Frontiers in plant science · 2013Article
- Plant-bacterium interactions analyzed by proteomics.Frontiers in plant science · 2013Article
- Recent advances in the composition and heterogeneity of the Arabidopsis mitochondrial proteome.Frontiers in plant science · 2013Article
- The extrafascicular phloem is made for fighting.Frontiers in plant science · 2013Article
- Deciphering systemic wound responses of the pumpkin extrafascicular phloem by metabolomics and stable isotope-coded protein labeling.Plant physiology · 2012Article
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7 authors.
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Abstract
The field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. Most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. In the human field, combinatorial hexapeptide ligand libraries (CPLL; such as ProteoMiner) have been used for reduction of the dynamic range of protein concentrations; however, this technique is not established in plant research. In this work, we present the application of CPLL to Arabidopsis (Arabidopsis thaliana) leaf proteins. One- and two-dimensional gel electrophoresis showed a decrease in high-abundance proteins and an enrichment of less abundant proteins in CPLL-treated samples. After optimization of the CPLL protocol, mass spectrometric analyses of leaf extracts led to the identification of 1,192 proteins in control samples and an additional 512 proteins after the application of CPLL. Upon leaf infection with virulent Pseudomonas syringae DC3000, CPLL beads were also used for investigating the bacterial infectome. In total, 312 bacterial proteins could be identified in infected Arabidopsis leaves. Furthermore, phloem exudates of pumpkin (Cucurbita maxima) were analyzed. CPLL prefractionation caused depletion of the major phloem proteins 1 and 2 and improved phloem proteomics, because 67 of 320 identified proteins were detectable only after CPLL treatment. In sum, our results demonstrate that CPLL beads are a time- and cost-effective tool for reducing major proteins, which often interfere with downstream analyses. The concomitant enrichment of less abundant proteins may facilitate a deeper insight into the plant proteome.
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