ArticleThe journal of nutrition, health & aging2012
Products of oxidized L-ascorbic acid damage acellular DNA.
Article in The journal of nutrition, health & aging, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed, 3 citations in OpenAlex.
- The pattern of RNA integrity and the expression of housekeeping genes are influenced by sodium hypochlorite and ascorbic acid.American journal of stem cells · 2023Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveL-ascorbic acid can be pro-oxidant and anti-oxidant in different reaction. This study aims to test the effects about products of oxidized L-Ascorbic Acid on acellular DNA. MEASUREMENT: Acellular DNA, nuclear DNA fixed on slides, are used in our experiment. There are four groups and one negative. Negative control is sham-treated with buffer(pH 7.2 and AA/ H2O2/fenton free). Experimental groups are treated separately with 0.06 mM L-ascorbic acid (AA) alone(exposed in air), 0.06 mM L-ascorbic acid (AA) alone(no exposure in air), 1.2 mM hydrogen peroxide (H2O2) alone, and a mixture of final concentration of 0.03 mM L-ascorbic acid and 0. 6 mM hydrogen peroxide (AA+ H2O2). Each experimental group consists of 4 slides and each slide is treated for 4 hours at 4 °C in a dark place. The DNA damage is quantified by alkaline Comet Assay. The comet images are analysed by Comet A1.0 software. Differences among groups are compared with SPSS 11.0.
resultsDNA singlestrand breakage is found to be treatment-dependent in the following sequence: AA+ H2O2> AA(oxidized) > H2O2, AA(without oxidization) and Control.
conclusionAcellular DNA can tolerate the low concentration H2O2, but is sensitive to free radical. The results indicate that AA expose in air and mixture of AA and H2O2 can produce •OH and L-dehydroascorbate (DHA), •OH can damage DNA.
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