ArticleTraffic (Copenhagen, Denmark)2012
Snail destabilizes cell surface Crumbs3a.
Article in Traffic (Copenhagen, Denmark), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- A size filter at the Golgi regulates apical membrane protein sorting.Nature cell biology · 2024Article
- Glycobiology of the Epithelial to Mesenchymal Transition.Biomedicines · 2021Review
- Newly synthesized claudins but not occludin are added to the basal side of the tight junction.Molecular biology of the cell · 2019Article
- Prostacyclin and EMT Pathway Markers for Monitoring Response to Lung Cancer Chemoprevention.Cancer prevention research (Philadelphia, Pa.) · 2018Article
- EPEC effector EspF promotes Crumbs3 endocytosis and disrupts epithelial cell polarity.Cellular microbiology · 2017Article
- The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135.The Journal of cell biology · 2015Article
- CRB2 completes a fully expressed Crumbs complex in the Retinal Pigment Epithelium.Scientific reports · 2015Article
- Developmental regulation of apical endocytosis controls epithelial patterning in vertebrate tubular organs.Nature cell biology · 2015Article
- Roles for trafficking and O-linked glycosylation in the turnover of model cell surface proteins.The Journal of biological chemistry · 2014Article
- Membrane-initiated estradiol signaling of epithelial-mesenchymal transition-associated mechanisms through regulation of tight junctions in human breast cancer cells.Hormones & cancer · 2014Article
- Crumbs3 is essential for proper epithelial development and viability.Molecular and cellular biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
During epithelial to mesenchymal transition (EMT), cells modulate expression of proteins resulting in loss of apical-basal polarity. Effectors of this EMT switch target the polarity protein Crumbs3a, a small transmembrane protein that is essential for generation of the apical membrane and tight junctions of mammalian epithelial cells. We previously showed that the Crumbs3 gene is a direct target of transcriptional regulation by Snail, a potent inducer of EMT. However, Snail has also been shown to have multiple non-transcriptional roles, including regulation of cell adhesion, proliferation and survival. Using SNAP-tag labeling, we determined that cell surface Crumbs3a has a half-life of approximately 3 h and that this cell surface half-life is significantly reduced when EMT is induced by Snail. We further observe that Snail induces differential glycosylation of Crumbs3a, including sialylation, suggesting a mechanism by which Crumbs3a may be destabilized. These results indicate that Crumbs3a is a post-translational target of Snail, in addition to being a transcriptional target. We conclude that Snail's ability to post-translationally modify and destabilize Crumbs3a augments the depolarizing process of EMT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.