ArticleMolecular microbiology2012
A cationic lumen in the Wzx flippase mediates anionic O-antigen subunit translocation in Pseudomonas aeruginosa PAO1.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Structural insights into a citrate transporter that mediates aluminum tolerance in barley.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide.Open biology · 2025Article
- Bacterial Outer Membrane Polysaccharide Export (OPX) Proteins Occupy Three Structural Classes with Selective β-Barrel Porin Requirements for Polymer Secretion.Microbiology spectrum · 2022Article
- Structure and Mechanism of the Lipid Flippase MurJ.Annual review of biochemistry · 2022Review
- Bacterial Glycocalyx Integrity Impacts Tolerance ofBiomolecules · 2022Article
- Modulation of bacterial multicellularity via spatio-specific polysaccharide secretion.PLoS biology · 2020Article
- Visualizing conformation transitions of the Lipid II flippase MurJ.Nature communications · 2019Article
- Structural features underlying recognition and translocation of extracellular polysaccharides.Interface focus · 2019Review
- Loss of specificity variants of WzxC suggest that substrate recognition is coupled with transporter opening in MOP-family flippases.Molecular microbiology · 2018Article
- Progress in Our Understanding of Wzx Flippase for Translocation of Bacterial Membrane Lipid-Linked Oligosaccharide.Journal of bacteriology · 2018Review
- Synthesis of Rhizobial Exopolysaccharides and Their Importance for Symbiosis with Legume Plants.Genes · 2017Review
- Review
- Filling holes in peptidoglycan biogenesis of Escherichia coli.Current opinion in microbiology · 2016Review
- A Bacteriophage-Acquired O-Antigen Polymerase (Wzyβ) from P. aeruginosa Serotype O16 Performs a Varied Mechanism Compared to Its Cognate Wzyα.Frontiers in microbiology · 2016Article
- GtrA Protein Rv3789 Is Required for Arabinosylation of Arabinogalactan in Mycobacterium tuberculosis.Journal of bacteriology · 2015Article
- Lipid Flippases for Bacterial Peptidoglycan Biosynthesis.Lipid insights · 2015Review
- Challenges and perspectives in combinatorial assembly of novel exopolysaccharide biosynthesis pathways.Frontiers in microbiology · 2015Review
- The Polymerization of Aeromonas hydrophila AH-3 O-Antigen LPS: Concerted Action of WecP and Wzy.PloS one · 2015Article
- Charge requirements of lipid II flippase activity in Escherichia coli.Journal of bacteriology · 2014Article
- AlignMe--a membrane protein sequence alignment web server.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Heteropolymeric B-band O-antigen (O-Ag) biosynthesis in Pseudomonas aeruginosa PAO1 follows the Wzy-dependent pathway, beginning with translocation of undecaprenyl pyrophosphate-linked anionic O-Ag subunits (O units) from the inner to the outer leaflets of the inner membrane (IM). This translocation is mediated by the integral IM flippase Wzx. Through experimentally based and unbiased topological mapping, our group previously observed that Wzx possesses many charged and aromatic amino acid residues within its 12 transmembrane segments (TMS). Herein, site-directed mutagenesis targeting 102 residues was carried out on the TMS and loops of Wzx, followed by assessment of each construct's ability to restore B-band O-Ag production, identifying eight residues important for flippase function. The importance of various charged and aromatic residues was highlighted, predominantly within the TMS of the protein, revealing functional 'hotspots' within the flippase, particularly within TMS2 and TMS8. Construction of a tertiary structure homology model for Wzx indicated that TMS2 and TMS8 line a central cationic lumen. This is the first report to describe a charged flippase lumen for mediating anionic O-unit translocation across the hydrophobic IM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.