ArticleMolecular biology of the cell2012
Phosphorylation of Rab11-FIP2 regulates polarity in MDCK cells.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 33 citations in OpenAlex.
- Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier.Molecular neurodegeneration · 2023Article
- Rab11FIP1-deficient mice develop spontaneous inflammation and show increased susceptibility to colon damage.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- PKA-independent vasopressin signaling in renal collecting duct.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Inhibition of the miR-192/215-Rab11-FIP2 axis suppresses human gastric cancer progression.Cell death & disease · 2018Article
- Insane in the apical membrane: Trafficking events mediating apicobasal epithelial polarity during tube morphogenesis.Traffic (Copenhagen, Denmark) · 2018Review
- Membrane Transport across Polarized Epithelia.Cold Spring Harbor perspectives in biology · 2017Review
- Abnormal Rab11-Rab8-vesicles cluster in enterocytes of patients with microvillus inclusion disease.Traffic (Copenhagen, Denmark) · 2017Article
- Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition.Molecular biology of the cell · 2017Article
- Proteomics Screen Identifies Class I Rab11 Family Interacting Proteins as Key Regulators of Cytokinesis.Molecular and cellular biology · 2017Article
- Article
- Rab11-FIP1 phosphorylation by MARK2 regulates polarity in MDCK cells.Cellular logistics · 2017Article
- Rab11-FIP1C Is a Critical Negative Regulator in ErbB2-Mediated Mammary Tumor Progression.Cancer research · 2016Article
- Loss of MYO5B in mice recapitulates Microvillus Inclusion Disease and reveals an apical trafficking pathway distinct to neonatal duodenum.Cellular and molecular gastroenterology and hepatology · 2016Article
- Rab11-FIP1A regulates early trafficking into the recycling endosomes.Experimental cell research · 2016Article
- Recycling endosomes.Current opinion in cell biology · 2015Review
- Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.Nature neuroscience · 2015Article
- Rab11a regulates syntaxin 3 localization and microvillus assembly in enterocytes.Journal of cell science · 2015Article
- Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.PloS one · 2015Article
- Myosin Vb uncoupling from RAB8A and RAB11A elicits microvillus inclusion disease.The Journal of clinical investigation · 2014Article
- Rab14 regulation of claudin-2 trafficking modulates epithelial permeability and lumen morphogenesis.Molecular biology of the cell · 2014Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The Rab11 effector Rab11-family interacting protein 2 (Rab11-FIP2) regulates transcytosis through its interactions with Rab11a and myosin Vb. Previous studies implicated Rab11-FIP2 in the establishment of polarity in Madin-Darby canine kidney (MDCK) cells through phosphorylation of Ser-227 by MARK2. Here we examine the dynamic role of Rab11-FIP2 phosphorylation on MDCK cell polarity. Endogenous Rab11-FIP2 phosphorylated on Ser-227 coalesces on vesicular plaques during the reestablishment of polarity after either monolayer wounding or calcium switch. Whereas expression of the nonphosphorylatable Rab11-FIP2(S227A) elicits a loss in lumen formation in MDCK cell cysts grown in Matrigel, the putative pseudophosphorylated Rab11-FIP2(S227E) mutant induces the formation of cysts with multiple lumens. On permeable filters, Rab11-FIP2(S227E)-expressing cells exhibit alterations in the composition of both the adherens and tight junctions. At the adherens junction, p120 catenin and K-cadherin are retained, whereas the majority of the E-cadherin is lost. Although ZO-1 is retained at the tight junction, occludin is lost and the claudin composition is altered. Of interest, the effects of Rab11-FIP2 on cellular polarity did not involve myosin Vb or Rab11a. These results indicate that Ser-227 phosphorylation of Rab11-FIP2 regulates the composition of both adherens and tight junctions and is intimately involved in the regulation of polarity in epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.