ArticleJournal of cell science2012
Bud4 mediates the cell-type-specific assembly of the axial landmark in budding yeast.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Septin Organization and Dynamics for Budding Yeast Cytokinesis.Journal of fungi (Basel, Switzerland) · 2024Review
- Cdc42 couples septin recruitment to the axial landmark assembly via Axl2 in budding yeast.Journal of cell science · 2024Article
- Role of the anillin-like protein in growth of Cryptococcus neoformans at human host temperature.Fungal genetics and biology : FG & B · 2022Article
- Characterization of Bud3 domains sufficient for bud neck targeting inAccess microbiology · 2022Article
- Septin Assembly and Remodeling at the Cell Division Site During the Cell Cycle.Frontiers in cell and developmental biology · 2021Review
- Review
- Regulation of Cdc42 for polarized growth in budding yeast.Microbial cell (Graz, Austria) · 2020Review
- Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Fine-tuning the orientation of the polarity axis by Rga1, a Cdc42 GTPase-activating protein.Molecular biology of the cell · 2017Article
- The final cut: cell polarity meets cytokinesis at the bud neck in S. cerevisiae.Cellular and molecular life sciences : CMLS · 2016Review
- Identification of Cell Cycle Dependent Interaction Partners of the Septins by Quantitative Mass Spectrometry.PloS one · 2016Article
- Regulation of Cdc42 polarization by the Rsr1 GTPase and Rga1, a Cdc42 GTPase-activating protein, in budding yeast.Journal of cell science · 2015Article
- The anillin-related region of Bud4 is the major functional determinant for Bud4's function in septin organization during bud growth and axial bud site selection in budding yeast.Eukaryotic cell · 2015Article
- Septins and Generation of Asymmetries in Fungal Cells.Annual review of microbiology · 2015Review
- Off-target effects of the septin drug forchlorfenuron on nonplant eukaryotes.Eukaryotic cell · 2014Article
- Sequential logic of polarity determination during the haploid-to-diploid transition in Saccharomyces cerevisiae.Eukaryotic cell · 2014Article
- Bud3 activates Cdc42 to establish a proper growth site in budding yeast.The Journal of cell biology · 2014Article
- Ubiquitin ligase trapping identifies an SCF(Saf1) pathway targeting unprocessed vacuolar/lysosomal proteins.Molecular cell · 2014Article
- Coupling of septins to the axial landmark by Bud4 in budding yeast.Journal of cell science · 2013Article
- Role of endocytosis in localization and maintenance of the spatial markers for bud-site selection in yeast.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Cell polarization occurs along a single axis that is generally determined by a spatial cue. Cells of the budding yeast Saccharomyces cerevisiae select a site for polarized growth in a specific pattern depending on cell type. Haploid a and α cells bud in the axial budding pattern, which depends on a transient marker and requires proteins Bud3, Bud4, Axl1 and Axl2. Here, we report that Bud4 functions as a platform that mediates the ordered assembly of the axial landmark at the division site during M and early G1 phase. Whereas Bud4 associates with Bud3 in all cell types and in the absence of Axl1 or Axl2, Bud4 interacts with Axl1 and Axl2 mainly in haploid cells and only in the presence of all other components of the landmark. Bud4 can bind to GTP or GDP, and a GTP-binding-defective Bud4 fails to interact with Axl1 in vitro. The same bud4 mutation leads to mis-localization of Axl1 and disrupts the axial budding pattern, indicating that GTP binding to Bud4 is important for its role in bud-site selection. We also show the cell-type-specific association of the axial landmark with Bud5, a GDP/GTP exchange factor for Rsr1. Despite their expression in all cell types, Bud4 and Axl2 associate with Bud5 specifically in haploid cells and in the presence of Axl1, whose expression is limited to a and α cells. Together, our findings suggest that Bud4 plays a critical role in the assembly of the axial landmark and its link to the Rsr1 GTPase module.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.