ArticleAnnals of botany2012
Composite Cucurbita pepo plants with transgenic roots as a tool to study root development.
Article in Annals of botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Composite Cucurbitaceae Plants as a Tool to Study Physiological and Molecular Mechanisms of Root Development.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Do DEEPER ROOTING 1 Homologs Regulate the Lateral Root Slope Angle in Cucumber (International journal of molecular sciences · 2024Article
- Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato.Plants (Basel, Switzerland) · 2023Article
- Lateral Root Initiation in Cucumber (International journal of molecular sciences · 2023Article
- Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots.International journal of molecular sciences · 2023Article
- A mutation in THREONINE SYNTHASE 1 uncouples proliferation and transition domains of the root apical meristem: experimental evidence and in silico proposed mechanism.Development (Cambridge, England) · 2022Article
- A polygalacturonase gene PG031 regulates seed coat permeability with a pleiotropic effect on seed weight in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation.Plants (Basel, Switzerland) · 2021Review
- One-step generation of composite soybean plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation.BMC plant biology · 2020Article
- Accumulation of and Response to Auxins in Roots and Nodules of the Actinorhizal PlantFrontiers in plant science · 2019Article
- Lateral Root Initiation in the Parental Root Meristem of Cucurbits: Old Players in a New Position.Frontiers in plant science · 2019Article
- Lateral root initiation and formation within the parental root meristem of Cucurbita pepo: is auxin a key player?Annals of botany · 2018Article
- Metabolic Engineering of Glycyrrhizin Pathway by Over-Expression of Beta-amyrin 11-Oxidase in Transgenic Roots of Glycyrrhiza glabra.Molecular biotechnology · 2018Article
- Generation of composite Persea americana (Mill.) (avocado) plants: A proof-of-concept-study.PloS one · 2017Article
- Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA.Plant cell reports · 2016Article
- Inhibition of auxin signaling in Frankia species-infected cells in Casuarina glauca nodules leads to increased nodulation.Plant physiology · 2015Article
- The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore-derived embryos.Protoplasma · 2014Article
- Ectopic expression of miR160 results in auxin hypersensitivity, cytokinin hyposensitivity, and inhibition of symbiotic nodule development in soybean.Plant physiology · 2013Article
- Plant root research: the past, the present and the future.Annals of botany · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsIn most plant species, initiation of lateral root primordia occurs above the elongation zone. However, in cucurbits and some other species, lateral root primordia initiation and development takes place in the apical meristem of the parental root. Composite transgenic plants obtained by Agrobacterium rhizogenes-mediated transformation are known as a suitable model to study root development. The aim of the present study was to establish this transformation technique for squash.
methodsThe auxin-responsive promoter DR5 was cloned into the binary vectors pKGW-RR-MGW and pMDC162-GFP. Incorporation of 5-ethynyl-2'-deoxyuridine (EdU) was used to evaluate the presence of DNA-synthesizing cells in the hypocotyl of squash seedlings to find out whether they were suitable for infection. Two A. rhizogenes strains, R1000 and MSU440, were used. Roots containing the respective constructs were selected based on DsRED1 or green fluorescent protein (GFP) fluorescence, and DR5::Egfp-gusA or DR5::gusA insertion, respectively, was verified by PCR. Distribution of the response to auxin was visualized by GFP fluorescence or β-glucuronidase (GUS) activity staining and confirmed by immunolocalization of GFP and GUS proteins, respectively. KEY
resultsBased on the distribution of EdU-labelled cells, it was determined that 6-day-old squash seedlings were suited for inoculation by A. rhizogenes since their root pericycle and the adjacent layers contain enough proliferating cells. Agrobacterium rhizogenes R1000 proved to be the most virulent strain on squash seedlings. Squash roots containing the respective constructs did not exhibit the hairy root phenotype and were morphologically and structurally similar to wild-type roots.
conclusionsThe auxin response pattern in the root apex of squash resembled that in arabidopsis roots. Composite squash plants obtained by A. rhizogenes-mediated transformation are a good tool for the investigation of root apical meristem development and root branching.
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