ArticleClinical oral investigations2013
Gene array of primary human osteoblasts exposed to enamel matrix derivative in combination with a natural bone mineral.
Article in Clinical oral investigations, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Combination of Enamel Matrix Derivatives with Bone Graft vs Bone Graft Alone in the Treatment of Periodontal Intrabony and Furcation Defects: A Systematic Review and Meta-Analysis.Oral health & preventive dentistry · 2024Pooled it
- TGF-β Signalling Mediates the Anti-Inflammatory Activity of Enamel Matrix Derivative In Vitro.International journal of molecular sciences · 2022Article
- Matrix mineralization controls gene expression in osteoblastic cells.Experimental cell research · 2018Article
- Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro.International journal of oral science · 2018Article
- Pre-coating deproteinized bovine bone mineral (DBBM) with bone-conditioned medium (BCM) improves osteoblast migration, adhesion, and differentiation in vitro.Clinical oral investigations · 2016Article
- Osteoinductive potential of 4 commonly employed bone grafts.Clinical oral investigations · 2016Article
- Gene array of PDL cells exposed to Osteogain in combination with a bone grafting material.Clinical oral investigations · 2016Article
- Bone grafting material in combination with Osteogain for bone repair: a rat histomorphometric study.Clinical oral investigations · 2016Article
- Effect of bone graft density on in vitro cell behavior with enamel matrix derivative.Clinical oral investigations · 2015Article
- Enamel matrix derivative promote primary human pulp cell differentiation and mineralization.International journal of molecular sciences · 2014Article
- In vitro-activity of oily calcium hydroxide suspension on microorganisms as well as on human alveolar osteoblasts and periodontal ligament fibroblasts.BMC oral health · 2014Article
- Effects of enamel matrix proteins in combination with a bovine-derived natural bone mineral for the repair of bone defects.Clinical oral investigations · 2014Article
- Computational analysis reveals a successive adaptation of multiple inositol polyphosphate phosphatase 1 in higher organisms through evolution.Evolutionary bioinformatics online · 2014Article
- Influence of enamel matrix derivative on cells at different maturation stages of differentiation.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe application of an enamel matrix derivative (EMD) for regenerative periodontal surgery has been shown to promote formation of new cementum, periodontal ligament, and alveolar bone. In intrabony defects with a complicated anatomy, the combination of EMD with various bone grafting materials has resulted in additional clinical improvements, but the initial cellular response of osteoblasts coming in contact with these particles have not yet been fully elucidated. The objective of the present study was to evaluate the in vitro effects of EMD combined with a natural bone mineral (NBM) on a wide variety of genes, cytokines, and transcription factors and extracellular matrix proteins on primary human osteoblasts. MATERIAL AND
methodsPrimary human osteoblasts were seeded on NBM particles pre-coated with versus without EMD and analyzed for gene differences using a human osteogenesis gene super-array (Applied Biosystems). Osteoblast-related genes include those transcribed during bone mineralization, ossification, bone metabolism, cell growth and differentiation, as well as gene products representing extracellular matrix molecules, transcription factors, and cell adhesion molecules.
resultsEMD promoted gene expression of various osteoblast differentiation markers including a number of collagen types and isoforms, SMAD intracellular proteins, osteopontin, cadherin, alkaline phosphatase, and bone sialoprotein. EMD also upregulated a variety of growth factors including bone morphogenetic proteins, vascular endothelial growth factors, insulin-like growth factor, transforming growth factor, and their associated receptor proteins.
conclusionThe results from the present study demonstrate that EMD is capable of activating a wide variety of genes, growth factors, and cytokines when pre-coated onto NBM particles. CLINICAL RELEVANCE: The described in vitro effects of EMD on human primary osteoblasts provide further biologic support for the clinical application of a combination of EMD with NBM particles in periodontal and oral regenerative surgery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.