ArticleProceedings of the National Academy of Sciences of the United States of America2012
Error-prone mammalian female meiosis from silencing the spindle assembly checkpoint without normal interkinetochore tension.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
91 citing papers in PubMed, 1 synthesis or guideline pooled it, 162 citations in OpenAlex.
- Biomechanics and mechanical signaling in the ovary: a systematic review.Journal of assisted reproduction and genetics · 2018Pooled it
- Maternal age as a driver of genome instability: mechanisms linking aneuploidy, mutagenesis and mitochondrial dysfunction.Archives of toxicology · 2026Review
- Article
- Tuning Meiotic Checkpoints: Evolutionary Costs and Mechanistic Trade-Offs.Annual review of genetics · 2026Review
- Specialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategy.eLife · 2026Article
- Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation.Nature communications · 2025Article
- Impact of Maternal Age on the Repairing Capacity of Oocytes on Paternal DNA Damage.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Article
- Assessment of Chromosome Congression Defects in Mammalian Oocytes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Increased DNA damage in full-grown oocytes is correlated with diminished autophagy activation.Nature communications · 2024Article
- Chromosome Division in Early Embryos-Is Everything under Control? And Is the Cell Size Important?International journal of molecular sciences · 2024Review
- Can Nicotinamide Adenine Dinucleotide (NADAnimals : an open access journal from MDPI · 2024Review
- Role of spindle assembly checkpoint proteins in gametogenesis and embryogenesis.Frontiers in cell and developmental biology · 2024Review
- Elevated sperm DNA fragmentation is correlated with an increased chromosomal aneuploidy rate of miscarried conceptus in women of advanced age undergoing fresh embryo transfer cycle.Frontiers in endocrinology · 2024Article
- Early onset of APC/C activity renders SAC inefficient in mouse embryos.Frontiers in cell and developmental biology · 2024Article
- Spindle assembly checkpoint insensitivity allows meiosis-II despite chromosomal defects in aged eggs.EMBO reports · 2023Article
- Meiotic cells escape prolonged spindle checkpoint activity through kinetochore silencing and slippage.PLoS genetics · 2023Article
- The large cytoplasmic volume of oocyte.The Journal of reproduction and development · 2023Review
- Bypassing Mendel's First Law: Transmission Ratio Distortion in Mammals.International journal of molecular sciences · 2023Review
- Meiotic Cells Escape Prolonged Spindle Checkpoint Activity Through Premature Silencing and Slippage.bioRxiv : the preprint server for biology · 2023Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
It is well established that chromosome segregation in female meiosis I (MI) is error-prone. The acentrosomal meiotic spindle poles do not have centrioles and are not anchored to the cortex via astral microtubules. By Cre recombinase-mediated removal in oocytes of the microtubule binding site of nuclear mitotic apparatus protein (NuMA), which is implicated in anchoring microtubules at poles, we determine that without functional NuMA, microtubules lose connection to MI spindle poles, resulting in highly disorganized early spindle assembly. Subsequently, very long spindles form with hyperfocused poles. The kinetochores of homologs make attachments to microtubules in these spindles but with reduced tension between them and accompanied by alignment defects. Despite this, the spindle assembly checkpoint is normally silenced and the advance to anaphase I and first polar body extrusion takes place without delay. Females without functional NuMA in oocytes are sterile, producing aneuploid eggs with altered chromosome number. These findings establish that in mammalian MI, the spindle assembly checkpoint is unable to sustain meiotic arrest in the presence of one or few misaligned and/or misattached kinetochores with reduced interkinetochore tension, thereby offering an explanation for why MI in mammals is so error-prone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.