ArticleVeterinary immunology and immunopathology2012
Generation and characterization of monoclonal antibodies to equine NKp46.
Article in Veterinary immunology and immunopathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Characterization of Nasal Mucosal T Cells in Horses and Their Response to Equine Herpesvirus Type 1.Viruses · 2024Article
- Pseudorabies Virus Glycoproteins E and B Application in Vaccine and Diagnosis Kit Development.Vaccines · 2024Review
- Populations of NK Cells and Regulatory T Cells in the Endometrium of Cycling Mares-A Preliminary Study.Animals : an open access journal from MDPI · 2022Article
- Single-cell resolution landscape of equine peripheral blood mononuclear cells reveals diverse cell types including T-betBMC biology · 2021Article
- Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses.PloS one · 2018Article
- NCR1+ cells in dogs show phenotypic characteristics of natural killer cells.Veterinary research communications · 2015Article
- NKp46+ CD3+ cells: a novel nonconventional T cell subset in cattle exhibiting both NK cell and T cell features.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.Veterinary research · 2013Article
- Placentation in Equids.Advances in anatomy, embryology, and cell biologyArticle
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Authors and funding
4 authors.
Funding
Abstract
The immunoreceptor NKp46 is considered to be the most consistent marker of NK cells across mammalian species. Here, we use a recombinant NKp46 protein to generate a panel of monoclonal antibodies that recognize equine NKp46. The extracellular region of equine NKp46 was expressed with equine IL-4 as a recombinant fusion protein (rIL-4/NKp46) and used as an immunogen to generate mouse monoclonal antibodies (mAbs). MAbs were first screened by ELISA for an ability to recognize NKp46, but not IL-4, or the structurally related immunoreceptor CD16. Nine mAbs were selected and were shown to recognize full-length NKp46 expressed on the surface of transfected CHO cells as a GFP fusion protein. The mAbs recognized a population of lymphocytes by flow cytometric analysis that was morphologically similar to NKp46+ cells in humans and cattle. In a study using nine horses, representative mAb 4F2 labeled 0.8-2.1% PBL with a mean fluorescence intensity consistent with gene expression data. MAb 4F2+ PBL were enriched by magnetic cell sorting and were found to express higher levels of NKP46 mRNA than 4F2- cells by quantitative RT-PCR. CD3-depleted PBL from five horses contained a higher percentage of 4F2+ cells than unsorted PBL. Using ELISA, we determined that the nine mAbs recognize three different epitopes. These mAbs will be useful tools in better understanding the largely uncharacterized equine NK cell population.
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