ArticleBiomaterials2012
Characterization of natural, decellularized and reseeded porcine tooth bud matrices.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- BMP-7 Enhances the Angiogenic Potential of Wharton's Jelly Mesenchymal Stem Cells Seeded in Decellularized Human Dental Pulp.International journal of molecular sciences · 2026Article
- Dentinogenic Effect of BMP-7 on Wharton's Jelly Mesenchymal Stem Cells Cultured in Decellularized Dental Pulp.International journal of molecular sciences · 2025Article
- Decellularization of Rat Submandibular Gland for Salivary Gland Tissue-Engineering Applications.International dental journal · 2025Article
- In vivo bioengineered tooth formation using decellularized tooth bud extracellular matrix scaffolds.Stem cells translational medicine · 2025Article
- Periapical lesion-derived decellularized extracellular matrix as a potential solution for regenerative endodontics.Regenerative biomaterials · 2024Article
- Injectable Xenogeneic Dental Pulp Decellularized Extracellular Matrix Hydrogel Promotes Functional Dental Pulp Regeneration.International journal of molecular sciences · 2023Article
- Creating a Microenvironment to Give Wings to Dental Pulp Regeneration-Bioactive Scaffolds.Pharmaceutics · 2023Review
- Hard Dental Tissues Regeneration-Approaches and Challenges.Materials (Basel, Switzerland) · 2021Review
- Tooth Repair and Regeneration: Potential of Dental Stem Cells.Trends in molecular medicine · 2021Review
- Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix.Scientific reports · 2020Article
- Tooth Bioengineering and Regenerative Dentistry.Journal of dental research · 2019Article
- Advances and perspectives in tooth tissue engineering.Journal of tissue engineering and regenerative medicine · 2017Review
- Decellularized Tooth Bud Scaffolds for Tooth Regeneration.Journal of dental research · 2017Article
- Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration.BioMed research international · 2017Article
- Interphotoreceptor matrix-poly(ϵ-caprolactone) composite scaffolds for human photoreceptor differentiation.Journal of tissue engineering · 2014Article
- Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Dental tissue engineering efforts have yet to identify scaffolds that instruct the formation of bioengineered teeth of predetermined size and shape. Here we investigated whether extracellular matrix (ECM) molecules present in natural tooth scaffolds can provide insight on how to achieve this goal. We describe methods to effectively decellularize and demineralize porcine molar tooth buds, while preserving natural ECM protein gradients. Natural tooth ECM composition was assessed using histological and immunohistochemical (IHC) analyses of fibrillar and basement membrane proteins. Our results showed that Collagen I, Fibronectin, Collagen IV, and Laminin gradients were detected in natural tooth tissues, and retained in decellularized samples. Second harmonic generation (SHG) image analysis and 3D reconstructions were used to show that natural tooth tissue exhibited higher collagen fiber density, and less oriented and less organized collagen fibers, as compared to decellularized tooth tissue. We also found that reseeded decellularized tooth scaffolds exhibited distinctive collagen content and organization as compared to decelluarized scaffolds. Our results show that SHG allows for quantitative assessment of ECM features that are not easily characterized using traditional histological analyses. In summary, our results demonstrate the potential for natural decellularized molar tooth ECM to instruct dental cell matrix synthesis, and lay the foundation for future use of biomimetic scaffolds for dental tissue engineering applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.