ArticleCold Spring Harbor protocols2012
Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy (polTIRFM).
Article in Cold Spring Harbor protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 21 citations in OpenAlex.
- Torque-generating units of the bacterial flagellar motor are rotary motors.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Kinematics of the lever arm swing in myosin VI.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Internal and external components of the bacterial flagellar motor rotate as a unit.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Phosphorylation of myosin regulatory light chain has minimal effect on kinetics and distribution of orientations of cross bridges of rabbit skeletal muscle.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- Single-molecule FRET and linear dichroism studies of DNA breathing and helicase binding at replication fork junctions.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Tilting and wobble of myosin V by high-speed single-molecule polarized fluorescence microscopy.Biophysical journal · 2013Article
- Simple estimation of Förster Resonance Energy Transfer (FRET) orientation factor distribution in membranes.International journal of molecular sciences · 2012Article
- The polarized total internal reflection fluorescence microscopy (polTIRFM) processive motility assay for myosin V.Cold Spring Harbor protocols · 2012Article
- The polarized total internal reflection fluorescence microscopy (polTIRFM) twirling filament assay.Cold Spring Harbor protocols · 2012Article
- The acquisition and analysis of polarized total internal reflection fluorescence microscopy (polTIRFM) data.Cold Spring Harbor protocols · 2012Article
- Construction of flow chambers for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays.Cold Spring Harbor protocols · 2012Article
- Fluorescent labeling of calmodulin with bifunctional rhodamine.Cold Spring Harbor protocols · 2012Article
- Preparation of filamentous actin for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays.Cold Spring Harbor protocols · 2012Article
- Fluorescent labeling of myosin V for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays.Cold Spring Harbor protocols · 2012Article
- Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation.ACS nano · 2011Article
- Changepoint analysis for single-molecule polarized total internal reflection fluorescence microscopy experiments.Methods in enzymology · 2011Article
- Impact of emission anisotropy on fluorescence spectroscopy and FRET distance measurements.Biophysical journal · 2009Article
- Twirling of actin by myosins II and V observed via polarized TIRF in a modified gliding assay.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In this article, we describe methods to detect the spatial orientation and rotational dynamics of single molecules using polarized total internal reflection fluorescence microscopy (polTIRFM). polTIRFM determines the three-dimensional angular orientation and the extent of wobble of a fluorescent probe bound to the macromolecule of interest. We discuss single-molecule versus ensemble measurements, as well as single-molecule techniques for orientation and rotation, and fluorescent probes for orientation studies. Using calmodulin (CaM) as an example of a target protein, we describe a method for labeling CaM with bifunctional rhodamine (BR). We also describe the physical principles and experimental setup of polTIRFM. We conclude with a brief introduction to assays using polTIRFM to assess the interaction of actin and myosin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.