ArticleJournal of lipid research2012
Evidence for the importance of OxPAPC interaction with cysteines in regulating endothelial cell function.
Article in Journal of lipid research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Oxidized phosphatidylcholines activate NOX1-mediated oxidative stress response and shift glucose metabolism in cardiac cells.American journal of physiology. Cell physiology · 2025Article
- Epigenetic silencing of interleukin-10 by host-derived oxidized phospholipids supports a lethal inflammatory response to infections.Immunity · 2025Article
- Dooming Phagocyte Responses: Inflammatory Effects of Endogenous Oxidized Phospholipids.Frontiers in endocrinology · 2021Review
- Innate Immune Interference Attenuates Inflammation In Bacillus Endophthalmitis.Investigative ophthalmology & visual science · 2020Article
- Role of Heparin-Binding Epidermal Growth Factor-Like Growth Factor in Oxidative Stress-Associated Metabolic Diseases.Metabolic syndrome and related disorders · 2020Review
- The effect of oxidized phospholipids on phenotypic polarization and function of macrophages.Free radical biology & medicine · 2017Review
- Inflammatory pain control by blocking oxidized phospholipid-mediated TRP channel activation.Scientific reports · 2017Article
- Identification of Oxidized Phosphatidylinositols Present in OxLDL and Human Atherosclerotic Plaque.Lipids · 2017Article
- An endogenous caspase-11 ligand elicits interleukin-1 release from living dendritic cells.Science (New York, N.Y.) · 2016Article
- Oxidized Phospholipid OxPAPC Activates TRPA1 and Contributes to Chronic Inflammatory Pain in Mice.PloS one · 2016Article
- Txnip ablation reduces vascular smooth muscle cell inflammation and ameliorates atherosclerosis in apolipoprotein E knockout mice.Atherosclerosis · 2015Article
- HDL inhibits the effects of oxidized phospholipids on endothelial cell gene expression via multiple mechanisms.Journal of lipid research · 2014Article
- Fatty acid epoxyisoprostane E2 stimulates an oxidative stress response in endothelial cells.Biochemical and biophysical research communications · 2014Article
- Phosphoproteomic analysis of platelets activated by pro-thrombotic oxidized phospholipids and thrombin.PloS one · 2014Article
- An epoxyisoprostane is a major regulator of endothelial cell function.Journal of medicinal chemistry · 2013Article
- High-density lipoprotein and 4F peptide reduce systemic inflammation by modulating intestinal oxidized lipid metabolism: novel hypotheses and review of literature.Arteriosclerosis, thrombosis, and vascular biology · 2012Review
- Role of phospholipid oxidation products in atherosclerosis.Circulation research · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Oxidation products of 1-palmitoyl-2-arachidonoyl-sn-glycerol-3-phosphatidylcholine (PAPC), referred to as OxPAPC, and an active component, 1-palmitoyl-2-(5,6-epoxyisoprostane E₂)-sn-glycero-3-phosphatidylcholine (PEIPC), accumulate in atherosclerotic lesions and regulate over 1,000 genes in human aortic endothelial cells (HAEC). We previously demonstrated that OxPNB, a biotinylated analog of OxPAPC, covalently binds to a number of proteins in HAEC. The goal of these studies was to gain insight into the binding mechanism and determine whether binding regulates activity. In whole cells, N-acetylcysteine inhibited gene regulation by OxPAPC, and blocking cell cysteines with N-ethylmaleimide strongly inhibited the binding of OxPNB to HAEC proteins. Using MS, we demonstrate that most of the binding of OxPAPC to cysteine is mediated by PEIPC. We also show that OxPNB and PEIPE-NB, the analog of PEIPC, bound to a model protein, H-Ras, at cysteines previously shown to regulate activity in response to 15-deoxy-Δ12,14-prostaglandin J2 (15dPGJ₂). This binding was observed with recombinant protein and in cells overexpressing H-Ras. OxPAPC and PEIPC compete with OxPNB for binding to H-Ras. 15dPGJ₂ and OxPAPC increased H-Ras activity at comparable concentrations. Using microarray analysis, we demonstrate a considerable overlap of gene regulation by OxPAPC, PEIPC, and 15dPGJ₂ in HAEC, suggesting that some effects attributed to 15dPGJ₂ may also be regulated by PEIPC because both molecules accumulate in inflammatory sites. Overall, we provide evidence for the importance of OxPAPC-cysteine interactions in regulating HAEC function.
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