ArticleThe Journal of biological chemistry2012
The basic properties of the electronic structure of the oxygen-evolving complex of photosystem II are not perturbed by Ca2+ removal.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Current perspectives on proton and electron transfer pathways in photosystem II water oxidation.Plant & cell physiology · 2025Review
- Closing Kok's cycle of nature's water oxidation catalysis.Nature communications · 2024Article
- Dissecting the relative contribution of ECA3 and group 8/9 cation diffusion facilitators to manganese homeostasis inPlant direct · 2023Article
- Role of redox-inactive metals in controlling the redox potential of heterometallic manganese-oxido clusters.Photosynthesis research · 2021Article
- Water oxidation in photosystem II.Photosynthesis research · 2019Review
- Calcium, conformational selection, and redox-active tyrosine YZ in the photosynthetic oxygen-evolving cluster.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Proton Matrix ENDOR Studies on Ca2+-depleted and Sr2+-substituted Manganese Cluster in Photosystem II.The Journal of biological chemistry · 2015Article
- Removal of Ca(2+) from the Oxygen-Evolving Complex in Photosystem II Has Minimal Effect on the Mn4O5 Core Structure: A Polarized Mn X-ray Absorption Spectroscopy Study.The journal of physical chemistry. B · 2015Article
- Correlation between pH dependence of O2 evolution and sensitivity of Mn cations in the oxygen-evolving complex to exogenous reductants.Photosynthesis research · 2015Article
- Investigations of the effect of the non-manganese metal in heterometallic-oxido cluster models of the oxygen evolving complex of photosystem II: lanthanides as substitutes for calcium.Inorganic chemistry · 2015Article
- Light-dependent production of dioxygen in photosynthesis.Metal ions in life sciences · 2015Review
- Pulse electron paramagnetic resonance studies of the interaction of methanol with the S2 state of the Mn4O5Ca cluster of photosystem II.Biochemistry · 2014Article
- Mn4Ca cluster in photosynthesis: where and how water is oxidized to dioxygen.Chemical reviews · 2014Review
- Synthetic cluster models of biological and heterogeneous manganese catalysts for O2 evolution.Inorganic chemistry · 2013Article
- Structural changes of the oxygen-evolving complex in photosystem II during the catalytic cycle.The Journal of biological chemistry · 2013Article
- Reduction potentials of heterometallic manganese-oxido cubane complexes modulated by redox-inactive metals.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The structures of Arabidopsis Deg5 and Deg8 reveal new insights into HtrA proteases.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Biological water-oxidizing complex: a nano-sized manganese-calcium oxide in a protein environment.Photosynthesis research · 2012Review
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5 authors.
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Abstract
Ca(2+) is an integral component of the Mn(4)O(5)Ca cluster of the oxygen-evolving complex in photosystem II (PS II). Its removal leads to the loss of the water oxidizing functionality. The S(2)' state of the Ca(2+)-depleted cluster from spinach is examined by X- and Q-band EPR and (55)Mn electron nuclear double resonance (ENDOR) spectroscopy. Spectral simulations demonstrate that upon Ca(2+) removal, its electronic structure remains essentially unaltered, i.e. that of a manganese tetramer. No redistribution of the manganese valence states and only minor perturbation of the exchange interactions between the manganese ions were found. Interestingly, the S(2)' state in spinach PS II is very similar to the native S(2) state of Thermosynechococcus elongatus in terms of spin state energies and insensitivity to methanol addition. These results assign the Ca(2+) a functional as opposed to a structural role in water splitting catalysis, such as (i) being essential for efficient proton-coupled electron transfer between Y(Z) and the manganese cluster and/or (ii) providing an initial binding site for substrate water. Additionally, a novel (55)Mn(2+) signal, detected by Q-band pulse EPR and ENDOR, was observed in Ca(2+)-depleted PS II. Mn(2+) titration, monitored by (55)Mn ENDOR, revealed a specific Mn(2+) binding site with a submicromolar K(D). Ca(2+) titration of Mn(2+)-loaded, Ca(2+)-depleted PS II demonstrated that the site is reversibly made accessible to Mn(2+) by Ca(2+) depletion and reconstitution. Mn(2+) is proposed to bind at one of the extrinsic subunits. This process is possibly relevant for the formation of the Mn(4)O(5)Ca cluster during photoassembly and/or D1 repair.
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