ArticleThe EMBO journal2012
Regulation of ABCB1/PGP1-catalysed auxin transport by linker phosphorylation.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
66 citing papers in PubMed, 139 citations in OpenAlex.
- Linker domain Ser-phosphorylation as a putative modulator for ABCB alkaloid transporters.Planta · 2026Review
- Dual ubiquitin signalling by SLOMO controls AUX1 activity and turnover during root gravitropism.The EMBO journal · 2026Article
- Comprehensive Analysis of theBiology · 2025Article
- HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane.Nature communications · 2025Article
- A key residue of the extracellular gate provides quality control contributing to ABCG substrate specificity.Nature communications · 2025Article
- Message hidden in α-helices-toward a better understanding of plant ABCG transporters' multispecificity.Plant physiology · 2025Article
- Review
- ABA importers ABCG17 and ABCG18 redundantly regulate seed size in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1.Plant communications · 2025Article
- Antioxidants by nature: an ancient feature at the heart of flavonoids' multifunctionality.The New phytologist · 2025Article
- Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor.Antioxidants (Basel, Switzerland) · 2024Article
- Systematic Investigation of Aluminum Stress-Related Genes and Their Critical Roles in Plants.International journal of molecular sciences · 2024Review
- Indole-3-propionic acid regulates lateral root development by targeting auxin signaling iniScience · 2024Article
- Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses inPlants (Basel, Switzerland) · 2023Article
- Suppression ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- ABCB-mediated shootward auxin transport feeds into the root clock.EMBO reports · 2023Article
- Inventory of ATP-binding cassette proteins in Lithospermum erythrorhizon as a model plant producing divergent secondary metabolites.DNA research : an international journal for rapid publication of reports on genes and genomes · 2022Article
- Systems approaches reveal that ABCB and PIN proteins mediate co-dependent auxin efflux.The Plant cell · 2022Article
- Mutations that alter Arabidopsis flavonoid metabolism affect the circadian clock.The Plant journal : for cell and molecular biology · 2022Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 8 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polar transport of the plant hormone auxin is controlled by PIN- and ABCB/PGP-efflux catalysts. PIN polarity is regulated by the AGC protein kinase, PINOID (PID), while ABCB activity was shown to be dependent on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Using co-immunoprecipitation (co-IP) and shotgun LC-MS/MS analysis, we identified PID as a valid partner in the interaction with TWD1. In-vitro and yeast expression analyses indicated that PID specifically modulates ABCB1-mediated auxin efflux in an action that is dependent on its kinase activity and that is reverted by quercetin binding and thus inhibition of PID autophosphorylation. Triple ABCB1/PID/TWD1 co-transfection in tobacco revealed that PID enhances ABCB1-mediated auxin efflux but blocks ABCB1 in the presence of TWD1. Phospho-proteomic analyses identified S634 as a key residue of the regulatory ABCB1 linker and a very likely target of PID phosphorylation that determines both transporter drug binding and activity. In summary, we provide evidence that PID phosphorylation has a dual, counter-active impact on ABCB1 activity that is coordinated by TWD1-PID interaction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.