ArticleThe Plant cell2012
CDKF;1 and CDKD protein kinases regulate phosphorylation of serine residues in the C-terminal domain of Arabidopsis RNA polymerase II.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 83 citations in OpenAlex.
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Bromodomain-containing proteins interact with a non-canonical RNA polymerase II kinase to maintain gene expression upon heat stress.Nature plants · 2025Article
- Artificial miRNAs and target-mimics as potential tools for crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Unveiling the Movement of RanBP1 During the Cell Cycle and Its Interaction with a Cyclin-Dependent Kinase (CDK) in Plants.International journal of molecular sciences · 2024Article
- The general transcription factors (GTFs) of RNA polymerase II and their roles in plant development and stress responses.Critical reviews in biochemistry and molecular biology · 2024Review
- Cotranscriptional demethylation induces global loss of H3K4me2 from active genes in Arabidopsis.The EMBO journal · 2023Article
- microRNA biogenesis and stabilization in plants.Fundamental research · 2023Review
- Hyponastic Leaves 1 Interacts with RNA Pol II to Ensure Proper Transcription of MicroRNA Genes.Plant & cell physiology · 2023Article
- A complex role of Arabidopsis CDKD;3 in meiotic progression and cytokinesis.Plant direct · 2023Article
- Genome-wide identification, evolutionary and expression analysis of the cyclin-dependent kinase gene family in peanut.BMC plant biology · 2023Article
- Recent Insights into Plant miRNA Biogenesis: Multiple Layers of miRNA Level Regulation.Plants (Basel, Switzerland) · 2023Review
- microRNA production inFrontiers in plant science · 2023Review
- An insight into microRNA biogenesis and its regulatory role in plant secondary metabolism.Plant cell reports · 2022Review
- MiRNA fine tuning for crop improvement: using advance computational models and biotechnological tools.Molecular biology reports · 2022Review
- Regulation of small RNA-mediated high temperature stress responses in crop plants.Plant cell reports · 2022Review
- Review
- Mechanisms of MicroRNA Biogenesis and Stability Control in Plants.Frontiers in plant science · 2022Review
- Recent advances in the regulation of plant miRNA biogenesis.RNA biology · 2021Review
- Serrate-Associated Protein 1, a splicing-related protein, promotes miRNA biogenesis in Arabidopsis.The New phytologist · 2021Article
- In Silico and Cellular Differences Related to the Cell Division Process between the A and B Races of the Colonial MicroalgaBiomolecules · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphorylation of conserved Y₁S₂P₃T₄S₅P₆S₇ repeats in the C-terminal domain of largest subunit of RNA polymerase II (RNAPII CTD) plays a central role in the regulation of transcription and cotranscriptional RNA processing. Here, we show that Ser phosphorylation of Arabidopsis thaliana RNAPII CTD is governed by CYCLIN-DEPENDENT KINASE F;1 (CDKF;1), a unique plant-specific CTD S₇-kinase. CDKF;1 is required for in vivo activation of functionally redundant CYCLIN-DEPENDENT KINASE Ds (CDKDs), which are major CTD S₅-kinases that also phosphorylate in vitro the S₂ and S₇ CTD residues. Inactivation of CDKF;1 causes extreme dwarfism and sterility. Inhibition of CTD S₇-phosphorylation in germinating cdkf;1 seedlings is accompanied by 3'-polyadenylation defects of pre-microRNAs and transcripts encoding key regulators of small RNA biogenesis pathways. The cdkf;1 mutation also decreases the levels of both precursor and mature small RNAs without causing global downregulation of the protein-coding transcriptome and enhances the removal of introns that carry pre-microRNA stem-loops. A triple cdkd knockout mutant is not viable, but a combination of null and weak cdkd;3 alleles in a triple cdkd123* mutant permits semidwarf growth. Germinating cdkd123* seedlings show reduced CTD S₅-phosphorylation, accumulation of uncapped precursor microRNAs, and a parallel decrease in mature microRNA. During later development of cdkd123* seedlings, however, S₇-phosphorylation and unprocessed small RNA levels decline similarly as in the cdkf;1 mutant. Taken together, cotranscriptional processing and stability of a set of small RNAs and transcripts involved in their biogenesis are sensitive to changes in the phosphorylation of RNAPII CTD by CDKF;1 and CDKDs.
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