ArticleThe Journal of general physiology2012
The intrinsic energy of the gating isomerization of a neuromuscular acetylcholine receptor channel.
Article in The Journal of general physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Dynamics of receptor activation by agonists.Biophysical journal · 2024Review
- Mechanism of hydrophobic gating in the acetylcholine receptor channel pore.The Journal of general physiology · 2024Article
- Article
- Derepression may masquerade as activation in ligand-gated ion channels.Nature communications · 2023Article
- Ancestral acetylcholine receptor β-subunit forms homopentamers that prime before opening spontaneously.eLife · 2022Article
- Agonist efficiency from concentration-response curves: Structural implications and applications.Biophysical journal · 2021Article
- Pathways for nicotinic receptor desensitization.The Journal of general physiology · 2020Article
- CHRFAM7A: A human specific fusion gene, accounts for the translational gap for cholinergic strategies in Alzheimer's disease.EBioMedicine · 2020Article
- Efficiency measures the conversion of agonist binding energy into receptor conformational change.The Journal of general physiology · 2019Article
- A single molecular distance predicts agonist binding energy in nicotinic receptors.The Journal of general physiology · 2019Article
- iPSC model of CHRFAM7A effect on α7 nicotinic acetylcholine receptor function in the human context.Translational psychiatry · 2019Article
- Mechanisms Underlying the Strong Inhibition of Muscle-Type Nicotinic Receptors by Tetracaine.Frontiers in molecular neuroscience · 2018Article
- Cyclic activation of endplate acetylcholine receptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Monod-Wyman-Changeux Analysis of Ligand-Gated Ion Channel Mutants.The journal of physical chemistry. B · 2017Article
- Choline and acetylcholine: what a difference an acetate makes!The Journal of physiology · 2017Article
- Molecular recognition at cholinergic synapses: acetylcholine versus choline.The Journal of physiology · 2017Article
- Structural correlates of affinity in fetal versus adult endplate nicotinic receptors.Nature communications · 2016Article
- Dose-Response Analysis When There Is a Correlation between Affinity and Efficacy.Molecular pharmacology · 2016Article
- Modal gating of endplate acetylcholine receptors: A proposed mechanism.The Journal of general physiology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nicotinic acetylcholine receptor (AChR) channels at neuromuscular synapses rarely open in the absence of agonists, but many different mutations increase the unliganded gating equilibrium constant (E0) to generate AChRs that are active constitutively. We measured E0 for two different sets of mutant combinations and by extrapolation estimated E0 for wild-type AChRs. The estimates were 7.6 and 7.8×10(-7) in adult-type mouse AChRs (-100 mV at 23°C). The values are in excellent agreement with one obtained previously by using a completely different method (6.5×10(-7), from monoliganded gating). E0 decreases with depolarization to the same extent as does the diliganded gating equilibrium constant, e-fold with ∼60 mV. We estimate that at -100 mV the intrinsic energy of the unliganded gating isomerization is +8.4 kcal/mol (35 kJ/mol), and that in the absence of a membrane potential, the intrinsic chemical energy of this global conformational change is +9.4 kcal/mol (39 kJ/mol). Na+ and K+ in the extracellular solution have no measureable effect on E0, which suggests that unliganded gating occurs with only water occupying the transmitter binding sites. The results are discussed with regard to the energy changes in receptor activation and the competitive antagonism of ions in agonist binding.
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