ArticleThe Journal of cell biology2012
Nonpolarized signaling reveals two distinct modes of 3D cell migration.
Article in The Journal of cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 216 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
216 citing papers in PubMed, 1 synthesis or guideline pooled it, 366 citations in OpenAlex.
- A meta-analysis indicates that the regulation of cell motility is a non-intrinsic function of chemoattractant receptors that is governed independently of directional sensing.Frontiers in immunology · 2022Pooled it
- Tissue flow acts as a guidance cue for immune cell polarization and directional migration.Nature cell biology · 2026Article
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- Spatio-temporal multiscale cell modeling of skin wound healing.Biophysical journal · 2026Article
- Human Cell-Derived Extracellular Matrix Modulates Endothelial Cell Morphology and Metabolism in Response to Fluid Shear Stress.ACS biomaterials science & engineering · 2026Article
- Nuclear Mechanics and Nuclear Mechanotransduction in Cancer Cell Migration and Invasion.Biomolecules · 2026Review
- Article
- Monocytes use protrusive forces to generate migration paths in viscoelastic collagen-based extracellular matrices.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Unjamming Transition as a Paradigm for Biomechanical Control of Cancer Metastasis.Cytoskeleton (Hoboken, N.J.) · 2025Review
- Representation of high-dimensional cell morphology and morphodynamics in 2D latent space.Physical biology · 2025Article
- Regulation of cell migration by extracellular matrix mechanics at a glance.Journal of cell science · 2025Review
- The role of dynamic reciprocity in 3D cell migration: connecting cell and matrix mechanics to migratory plasticity.npj biological physics and mechanics · 2025Review
- Physical principles and mechanisms of cell migration.npj biological physics and mechanics · 2025Review
- Coupled Dynamics in Phenotype and Tissue Spaces Shape the Three-Dimensional Cancer Invasion.PRX life · 2024Article
- A nanoporous hydrogel-based model to study chemokine gradient-driven angiogenesis under luminal flow.Lab on a chip · 2024Article
- Proteolysis-free amoeboid migration of melanoma cells through crowded environments via bleb-driven worrying.Developmental cell · 2024Article
- Dendritic cell force-migration coupling on aligned fiber networks.Biophysical journal · 2024Article
- Matrix stiffness-dependent regulation of immunomodulatory genes in human MSCs is associated with the lncRNA CYTOR.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A homeostatic role of nucleus-actin filament coupling in the regulation of cellular traction forces in fibroblasts.Biomechanics and modeling in mechanobiology · 2024Article
- Immune Cell Migration to Cancer.Cells · 2024Review
156 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on bySwitching to 3D.2012
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We search in this paper for context-specific modes of three-dimensional (3D) cell migration using imaging for phosphatidylinositol (3,4,5)-trisphosphate (PIP3) and active Rac1 and Cdc42 in primary fibroblasts migrating within different 3D environments. In 3D collagen, PIP3 and active Rac1 and Cdc42 were targeted to the leading edge, consistent with lamellipodia-based migration. In contrast, elongated cells migrating inside dermal explants and the cell-derived matrix (CDM) formed blunt, cylindrical protrusions, termed lobopodia, and Rac1, Cdc42, and PIP3 signaling was nonpolarized. Reducing RhoA, Rho-associated protein kinase (ROCK), or myosin II activity switched the cells to lamellipodia-based 3D migration. These modes of 3D migration were regulated by matrix physical properties. Specifically, experimentally modifying the elasticity of the CDM or collagen gels established that nonlinear elasticity supported lamellipodia-based migration, whereas linear elasticity switched cells to lobopodia-based migration. Thus, the relative polarization of intracellular signaling identifies two distinct modes of 3D cell migration governed intrinsically by RhoA, ROCK, and myosin II and extrinsically by the elastic behavior of the 3D extracellular matrix.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.