ArticleMolecular biology reports2012
Induction and activation of antiviral enzyme 2',5'-oligoadenylate synthetase by in vitro transcribed insulin mRNA and other cellular RNAs.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- The Enzymatic Mechanism of OAS: How Metal Ions and Quantum Effects Help Activate Innate Immunity.ACS omega · 2026Article
- Redox regulation of mNature cell biology · 2024Article
- piR-36249 and DHX36 together inhibit testicular cancer cells progression by upregulating OAS2.Non-coding RNA research · 2023Article
- Epigenetics in psoriasis: perspective of DNA methylation.Molecular genetics and genomics : MGG · 2021Review
- Intracellular Routing and Recognition of Lipid-Based mRNA Nanoparticles.Pharmaceutics · 2021Review
- Alternative splicing coupled with transcript degradation modulates OAS1g antiviral activity.RNA (New York, N.Y.) · 2020Article
- Recurrent Loss-of-Function Mutations Reveal Costs to OAS1 Antiviral Activity in Primates.Cell host & microbe · 2019Article
- Opposite functions of GSN and OAS2 on colorectal cancer metastasis, mediating perineural and lymphovascular invasion, respectively.PloS one · 2018Article
- Epigenetic regulation of OAS2 shows disease-specific DNA methylation profiles at individual CpG sites.Scientific reports · 2016Article
- Structural mechanism of sensing long dsRNA via a noncatalytic domain in human oligoadenylate synthetase 3.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
Double-stranded RNA (dsRNA) can induce antiviral enzyme 2',5'-oligoadenylate synthetase (2'5'AS) expression and activate latent 2'5'AS. Our previous data have shown pancreatic β cells are sensitive to dsRNA-induced 2'5'AS expression, and constitutive high basal 2'5'AS expression is associated with susceptibility to developing type 1 diabetes (T1D), a disease due to pancreatic β cell loss. Here we report that in vitro transcribed human insulin mRNA induces the activation of human OAS gene promoter sequences, and specifically and dose-dependently induces 2'5'AS expression in murine pancreatic βTC3 cells. Over-expression of dsRNA receptor retinoic acid-inducible gene-1 enhances insulin mRNA-induced 2'5'AS expression. In vitro transcribed insulin and other mRNAs, as well as total cellular RNAs, activate latent 2'5'AS in vitro with activation ability likely associated with the sequence and length of individual mRNAs or the sample source of total cellular RNA. Insulin mRNA does not show any specificity to activate 2'5'AS, but total cellular RNA from βTC3 cells has high activation ability. Constitutive 2'5'AS expression in βTC3 cells leads to cell proliferation inhibition and apoptosis. Our study suggests the possibility of cellular RNA-regulated 2'5'AS expression and activation, and the potential risk of high insulin gene transcription in pancreatic β cells, and may help explain genetic predisposition to T1D associated with INS VNTR class I alleles.
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