ArticleJournal of visualized experiments : JoVE2012
Quantification of fungal colonization, sporogenesis, and production of mycotoxins using kernel bioassays.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
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- Role of the LAMMER kinase LkhA in fungal development and aflatoxin production in Aspergillus flavus.Journal of microbiology (Seoul, Korea) · 2025Article
- Characterization of Blue Light Receptors LreA and LreB inJournal of microbiology and biotechnology · 2025Article
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- Drought-induced assembly of rhizosphere mycobiomes shows beneficial effects on plant growth.mSystems · 2024Article
- FvKex2 is required for development, virulence, and mycotoxin production in Fusarium verticillioides.Applied microbiology and biotechnology · 2024Article
- Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance toJournal of fungi (Basel, Switzerland) · 2023Article
- The KdmB-EcoA-RpdA-SntB (KERS) chromatin regulatory complex controls development, secondary metabolism and pathogenicity in Aspergillus flavus.Fungal genetics and biology : FG & B · 2023Article
- Vacuole Proteins with Optimized Microtubule Assembly Is Required for Fum1 Protein Localization and Fumonisin Biosynthesis in Mycotoxigenic FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Physico-chemical characteristics and aflatoxins production of Atractylodis Rhizoma to different storage temperatures and humidities.AMB Express · 2021Article
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- Two regulators of G-protein signaling (RGS) proteins FlbA1 and FlbA2 differentially regulate fumonisin B1 biosynthesis in Fusarium verticillioides.Current genetics · 2021Article
- A Rab GTPase protein FvSec4 is necessary for fumonisin B1 biosynthesis and virulence in Fusarium verticillioides.Current genetics · 2020Article
- Function ofToxins · 2019Article
- Identification of a Novel Transcription Factor TP05746 Involved in Regulating the Production of Plant-Biomass-Degrading Enzymes inFrontiers in microbiology · 2019Article
- The epigenetic reader SntB regulates secondary metabolism, development and global histone modifications in Aspergillus flavus.Fungal genetics and biology : FG & B · 2018Article
- Oxylipins from both pathogen and host antagonize jasmonic acid-mediated defence via the 9-lipoxygenase pathway in Fusarium verticillioides infection of maize.Molecular plant pathology · 2018Article
- Fungal and herbivore elicitation of the novel maize sesquiterpenoid, zealexin A4, is attenuated by elevated COPlanta · 2018Article
- Contribution of ATPase copper transporters in animal but not plant virulence of the crossover pathogen Aspergillus flavus.Virulence · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rotting of grains by seed-infecting fungi poses one of the greatest economic challenges to cereal production worldwide, not to mention serious risks to human and animal health. Among cereal production, maize is arguably the most affected crop, due to pathogen-induced losses in grain integrity and mycotoxin seed contamination. The two most prevalent and problematic mycotoxins for maize growers and food and feed processors are aflatoxin and fumonisin, produced by Aspergillus flavus and Fusarium verticillioides, respectively. Recent studies in molecular plant-pathogen interactions have demonstrated promise in understanding specific mechanisms associated with plant responses to fungal infection and mycotoxin contamination(1,2,3,4,5,6). Because many labs are using kernel assays to study plant-pathogen interactions, there is a need for a standardized method for quantifying different biological parameters, so results from different laboratories can be cross-interpreted. For a robust and reproducible means for quantitative analyses on seeds, we have developed in-lab kernel assays and subsequent methods to quantify fungal growth, biomass, and mycotoxin contamination. Four sterilized maize kernels are inoculated in glass vials with a fungal suspension (10(6)) and incubated for a predetermined period. Sample vials are then selected for enumeration of conidia by hemocytometer, ergosterol-based biomass analysis by high performance liquid chromatography (HPLC), aflatoxin quantification using an AflaTest fluorometer method, and fumonisin quantification by HPLC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.