ArticleAsian Pacific journal of tropical medicine2012
Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus.
Article in Asian Pacific journal of tropical medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR).BMC infectious diseases · 2026Article
- Chikungunya virus in Thailand (2020-2023): Epidemiology, clinical features, and genomic insights.PLoS neglected tropical diseases · 2025Article
- Development of nanobody-based DAS-ELISA and Au nanoparticle-based immunochromatographic test strip for highly sensitive detection of Chikungunya virus.Frontiers in immunology · 2025Article
- Simultaneous detection and differentiation of dengue and chikungunya viruses for commercial utility.Virusdisease · 2023Article
- Molecular surveillance of arboviruses circulation and co-infection during a large chikungunya virus outbreak in Thailand, October 2018 to February 2020.Scientific reports · 2022Article
- Isolation and characterization of high affinity and highly stable anti-Chikungunya virus antibodies using ALTHEA Gold Libraries™.BMC infectious diseases · 2021Article
- Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses.Biosensors · 2021Article
- A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2020Article
- Article
- Beyond Fever and Pain: Diagnostic Methods for Chikungunya Virus.Journal of clinical microbiology · 2019Review
- Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.BMC bioinformatics · 2018Article
- A unique case of human Zika virus infection in association with severe liver injury and coagulation disorders.Scientific reports · 2017Article
- Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America.Diagnostic microbiology and infectious disease · 2017Article
- Chikungunya: important lessons from the Jamaican experience.Revista panamericana de salud publica = Pan American journal of public health · 2017Article
- Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing.Journal of clinical microbiology · 2017Article
- [Meta-analysis of the usefulness of ELISA, PCR, and immunochromatography for the diagnosis of ChikungunyaMeta-análise da utilidade dos exames ELISA, PCR e imunocromatografia no diagnóstico de febre chikungunya].Revista panamericana de salud publica = Pan American journal of public health · 2017Article
- Evaluation of the vector competence of a native UK mosquito Ochlerotatus detritus (Aedes detritus) for dengue, chikungunya and West Nile viruses.Parasites & vectors · 2016Article
- Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays.The American journal of tropical medicine and hygiene · 2016Article
- Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2016Article
- Spectrum of Viral Pathogens in Blood of Malaria-Free Ill Travelers Returning to Canada.Emerging infectious diseases · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.
methodsWe have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.
resultsThe assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus.
conclusionsThis rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.
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