ArticleMicrobial cell factories2012
De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia lipolytica.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 73 citations in OpenAlex.
- Activity-based protein profiling guided new target identification of quinazoline derivatives for expediting bactericide discovery: Activity-based protein profiling derived new target discovery of antibacterial quinazolines.Journal of advanced research · 2025Article
- Biosynthesis of conjugated linoleic acid: current status and future perspectives.Bioresources and bioprocessing · 2025Review
- Proteomics and Metabolomics Analysis Reveal the Regulation Mechanism of Linoleate Isomerase Activity and Function inACS omega · 2024Article
- Biosynthesis of Fatty Acid Derivatives by RecombinantJournal of fungi (Basel, Switzerland) · 2023Article
- EngineeringSynthetic and systems biotechnology · 2022Review
- Molecular mechanism of interaction between fatty acid delta 6 desaturase and acyl-CoA by computational prediction.AMB Express · 2022Article
- Enhanced Soluble Expression of Linoleic Acid Isomerase by Coordinated Regulation of Promoter and Fusion Tag inFoods (Basel, Switzerland) · 2022Article
- Metabolic Engineering for Unusual Lipid Production inMicroorganisms · 2020Review
- Review
- Modulating Heterologous Pathways and Optimizing Culture Conditions for Biosynthesis ofMolecules (Basel, Switzerland) · 2019Article
- Synthetic biology tools for engineering Yarrowia lipolytica.Biotechnology advances · 2018Review
- Simple glycolipids of microbes: Chemistry, biological activity and metabolic engineering.Synthetic and systems biotechnology · 2018Review
- A metabolic engineering strategy for producing conjugated linoleic acids using the oleaginous yeast Yarrowia lipolytica.Applied microbiology and biotechnology · 2017Article
- Lipid biosynthesis in yeasts: A comparison of the lipid biosynthetic pathway between the model nonoleaginous yeastEngineering in life sciences · 2017Review
- Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2015Article
- Protein production in Yarrowia lipolytica via fusion to the secreted lipase Lip2p.Molecular biotechnology · 2014Article
- A novel multigene expression construct for modification of glycerol metabolism in Yarrowia lipolytica.Microbial cell factories · 2013Article
- Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial applications.Journal of industrial microbiology & biotechnology · 2013Article
- Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10, cis-12 conjugated linoleic acid.Microbial cell factories · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConjugated linoleic acid (CLA) has many well-documented beneficial physiological effects. Due to the insufficient natural supply of CLA and low specificity of chemically produced CLA, an effective and isomer-specific production process is required for medicinal and nutritional purposes.
resultsThe linoleic acid isomerase gene from Propionibacterium acnes was expressed in Yarrowia lipolytica Polh. Codon usage optimization of the PAI and multi-copy integration significantly improved the expression level of PAI in Y. lipolytica. The percentage of trans-10, cis-12 CLA was six times higher in yeast carrying the codon-optimized gene than in yeast carrying the native gene. In combination with multi-copy integration, the production yield was raised to approximately 30-fold. The amount of trans-10, cis-12 CLA reached 5.9% of total fatty acid yield in transformed Y. lipolytica.
conclusionsThis is the first report of production of trans-10, cis-12 CLA by the oleaginous yeast Y. lipolytica, using glucose as the sole carbon source through expression of linoleic acid isomerase from Propionibacterium acnes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.