ArticlePloS one2012
Structure of an enzyme-derived phosphoprotein recognition domain.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Molecular and evolutionary determinants for protein interaction within a class II aldolase/Adducin domain.PloS one · 2025Article
- Metamorphic protein folding as evolutionary adaptation.Trends in biochemical sciences · 2023Review
- The Drosophila mitotic spindle orientation machinery requires activation, not just localization.EMBO reports · 2023Article
- Article
- Entropy of stapled peptide inhibitors in free state is the major contributor to the improvement of binding affinity with the GK domain.RSC chemical biology · 2021Article
- The Case of the Scribble Polarity Module in Asymmetric Neuroblast Division in Development and Tumorigenesis.International journal of molecular sciences · 2020Review
- Evolution of a Protein Interaction Domain Family by Tuning Conformational Flexibility.Journal of the American Chemical Society · 2016Article
- Regulation of mitotic spindle orientation: an integrated view.EMBO reports · 2016Review
- Pins is not required for spindle orientation in the Drosophila wing disc.Development (Cambridge, England) · 2016Article
- Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation.Nature structural & molecular biology · 2016Article
- Evolution of an ancient protein function involved in organized multicellularity in animals.eLife · 2016Article
- Spatiotemporal phosphoregulation of Lgl: Finding meaning in multiple on/off buttons.Bioarchitecture · 2016Article
- Cell Fate Decision Making through Oriented Cell Division.Journal of developmental biology · 2015Article
- Epithelial homeostasis.Current biology : CB · 2014Review
- Phosphorylation-dependent interaction between tumor suppressors Dlg and Lgl.Cell research · 2014Article
- Dishevelled binds the Discs large 'Hook' domain to activate GukHolder-dependent spindle positioning in Drosophila.PloS one · 2014Article
- Discs large links spindle orientation to apical-basal polarity in Drosophila epithelia.Current biology : CB · 2013Article
- A NudE/14-3-3 pathway coordinates dynein and the kinesin Khc73 to position the mitotic spindle.Developmental cell · 2013Article
- Molecular pathways regulating mitotic spindle orientation in animal cells.Development (Cambridge, England) · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Membrane Associated Guanylate Kinases (MAGUKs) contain a protein interaction domain (GK(dom)) derived from the enzyme Guanylate Kinase (GK(enz)). Here we show that GK(dom) from the MAGUK Discs large (Dlg) is a phosphoprotein recognition domain, specifically recognizing the phosphorylated form of the mitotic spindle orientation protein Partner of Inscuteable (Pins). We determined the structure of the Dlg-Pins complex to understand the dramatic transition from nucleotide kinase to phosphoprotein recognition domain. The structure reveals that the region of the GK(dom) that once served as the GMP binding domain (GBD) has been co-opted for protein interaction. Pins makes significantly more contact with the GBD than does GMP, but primarily with residues that are conserved between enzyme and domain revealing the versatility of the GBD as a platform for nucleotide and protein interactions. Mutational analysis reveals that the GBD is also used to bind the GK ligand MAP1a, suggesting that this is a common mode of MAGUK complex assembly. The GK(enz) undergoes a dramatic closing reaction upon GMP binding but the protein-bound GK(dom) remains in the 'open' conformation indicating that the dramatic conformational change has been lost in the conversion from nucleotide kinase to phosphoprotein recognition domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.