ArticleJournal of neurovirology2012
Immunohistochemical detection of intra-neuronal VZV proteins in snap-frozen human ganglia is confounded by antibodies directed against blood group A1-associated antigens.
Article in Journal of neurovirology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Varicella-zoster virus proteome-wide T-cell screening demonstrates low prevalence of virus-specific CD8 T-cells in latently infected human trigeminal ganglia.Journal of neuroinflammation · 2023Article
- A Guide to Preclinical Models of Zoster-Associated Pain and Postherpetic Neuralgia.Current topics in microbiology and immunology · 2023Review
- Varicella Zoster Virus Neuronal Latency and Reactivation Modeled in Vitro.Current topics in microbiology and immunology · 2023Review
- Selective retention of virus-specific tissue-resident T cells in healed skin after recovery from herpes zoster.Nature communications · 2022Article
- No Evidence of Varicella-Zoster Virus Infection in Temporal Artery Biopsies of Anterior Ischemic Optic Neuropathy Patients With and Without Giant Cell Arteritis.The Journal of infectious diseases · 2021Article
- Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency.Nature communications · 2020Article
- Review
- Review
- Molecular Aspects of Varicella-Zoster Virus Latency.Viruses · 2018Review
- Simian Varicella Virus Infects Enteric Neurons and α4β7 Integrin-Expressing Gut-Tropic T-Cells in Nonhuman Primates.Viruses · 2018Article
- A spliced latency-associated VZV transcript maps antisense to the viral transactivator gene 61.Nature communications · 2018Article
- Patterns of BAP1 protein expression provide insights into prognostic significance and the biology of uveal melanoma.The journal of pathology. Clinical research · 2018Article
- In vitro system using human neurons demonstrates that varicella-zoster vaccine virus is impaired for reactivation, but not latency.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Neuronal changes induced by Varicella Zoster Virus in a rat model of postherpetic neuralgia.Virology · 2015Article
- A comparison of herpes simplex virus type 1 and varicella-zoster virus latency and reactivation.The Journal of general virology · 2015Review
- Pathogenesis of varicelloviruses in primates.The Journal of pathology · 2015Review
- Presence of a neo-epitope and absence of amyloid beta and tau protein in degenerative hippocampal granules of aged mice.Age (Dordrecht, Netherlands) · 2014Article
- Pathogenesis and current approaches to control of varicella-zoster virus infections.Clinical microbiology reviews · 2013Review
- T-cell immunity to human alphaherpesviruses.Current opinion in virology · 2013Review
- Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.Journal of virology · 2013Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Varicella-zoster virus (VZV) causes chickenpox, establishes latency in trigeminal (TG) and dorsal root ganglia (DRG), and can lead to herpes zoster upon reactivation. The VZV proteome expressed during latency remains ill-defined, and previous studies have shown discordant data on the spectrum and expression pattern of VZV proteins and transcripts in latently infected human ganglia. Recently, Zerboni and colleagues have provided new insight into this discrepancy (Zerboni et al. in J Virol 86:578-583, 2012). They showed that VZV-specific ascites-derived monoclonal antibody (mAb) preparations contain endogenous antibodies directed against blood group A1 proteins, resulting in false-positive intra-neuronal VZV staining in formalin-fixed human DRG. The aim of the present study was to confirm and extend this phenomenon to snap-frozen TG (n=30) and DRG (n=9) specimens of blood group genotyped donors (n=30). The number of immunohistochemically stained neurons was higher with mAb directed to immediate early protein 62 (IE62) compared with IE63. The IE63 mAb-positive neurons always co-stained for IE62 but not vice versa. The mAb staining was confined to distinct large intra-neuronal vacuoles and restricted to A1(POS) donors. Anti-VZV mAb staining in neurons, but not in VZV-infected cell monolayers, was obliterated after mAb adsorption against blood group A1 erythrocytes. The data presented demonstrate that neuronal VZV protein expression detected by ascites-derived mAb in snap-frozen TG and DRG of blood group A1(POS) donors can be misinterpreted due to the presence of endogenous antibodies directed against blood group A1-associated antigens present in ascites-derived VZV-specific mAb preparations.
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Registered trials
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