ArticleHistochemistry and cell biology2012
Plasmid DNA is internalized from the apical plasma membrane of the salivary gland epithelium in live animals.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Actin and Myosin in Non-Neuronal Exocytosis.Cells · 2020Review
- Application of advances in endocytosis and membrane trafficking to drug delivery.Advanced drug delivery reviews · 2020Review
- Intravital microscopy in mammalian multicellular organisms.Current opinion in cell biology · 2019Review
- Brain-Derived Neurotrophic Factor Loaded PS80 PBCA Nanocarrier for In Vitro Neural Differentiation of Mouse Induced Pluripotent Stem Cells.International journal of molecular sciences · 2017Article
- Imaging membrane remodeling during regulated exocytosis in live mice.Experimental cell research · 2015Review
- Purification and ex vivo expansion of fully functional salivary gland stem cells.Stem cell reports · 2014Article
- Imaging the dynamics of endocytosis in live mammalian tissues.Cold Spring Harbor perspectives in biology · 2014Review
- Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells.Molecular biotechnology · 2014Article
- Polyethylenimine-mediated expression of transgenes in the acinar cells of rats salivary glands in vivo.Frontiers in cell and developmental biology · 2014Article
- Imaging cell biology in live animals: ready for prime time.The Journal of cell biology · 2013Review
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- The Histochemistry and Cell Biology compendium: a review of 2012.Histochemistry and cell biology · 2013Review
- Article
- Intravital microscopy: a practical guide on imaging intracellular structures in live animals.BioarchitectureReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Non-viral-mediated gene delivery represents an alternative way to express the gene of interest without inducing immune responses or other adverse effects. Understanding the mechanisms by which plasmid DNAs are delivered to the proper target in vivo is a fundamental issue that needs to be addressed in order to design more effective strategies for gene therapy. As a model system, we have used the submandibular salivary glands in live rats and we have recently shown that reporter transgenes can be expressed in different cell populations of the glandular epithelium, depending on the modality of administration of plasmid DNA. Here, by using a combination of immunofluorescence and intravital microscopy, we have explored the relationship between the pattern of transgenes expression and the internalization of plasmid DNA. We found that plasmid DNA is internalized: (1) by all the cells in the salivary gland epithelium, when administered alone, (2) by large ducts, when mixed with empty adenoviral particles, and (3) by acinar cells upon stimulation of compensatory endocytosis. Moreover, we showed that plasmid DNA utilizes different routes of internalization, and evades both the lysosomal degradative pathway and the retrograde pathway towards the Golgi apparatus. This study clearly shows that in vivo approaches have the potential to address fundamental questions on the cellular mechanisms regulating gene delivery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.