ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2012
Pyrosequencing reveals the complex polymicrobial nature of invasive pyogenic infections: microbial constituents of empyema, liver abscess, and intracerebral abscess.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Brain microbial populations in HIV/AIDS: α-proteobacteria predominate independent of host immune status.PloS one · 2013Trial
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- Novel Use of Metagenomic Next-Generation Sequencing in DiagnosingInfection and drug resistance · 2024Article
- Importation of Entamoeba histolytica and predominance of Klebsiella pneumoniae in liver abscesses: a 7-year retrospective cohort study from the United Arab Emirates.Tropical diseases, travel medicine and vaccines · 2021Article
- Pathogen determination from clinical abscess fluids using metagenomic next-generation sequencing.Folia microbiologica · 2021Article
- From Normal Flora to Brain Abscesses: A Review ofFrontiers in microbiology · 2020Review
- Clinical characteristics and treatment outcomes in a cohort of patients with pyogenic and amoebic liver abscess.BMC infectious diseases · 2019Article
- Bacterial DNA patterns identified using paired-end Illumina sequencing of 16S rRNA genes from whole blood samples of septic patients in the emergency room and intensive care unit.BMC microbiology · 2018Article
- Molecular Identification of Bacteria in Intra-abdominal Abscesses Using Deep Sequencing.Open forum infectious diseases · 2018Article
- Fusobacterial liver abscess: a case report and review of the literature.BMC infectious diseases · 2017Review
- Combined Signature of the Fecal Microbiome and Metabolome in Patients with Gout.Frontiers in microbiology · 2017Article
- Automated Broad-Range Molecular Detection of Bacteria in Clinical Samples.Journal of clinical microbiology · 2016Article
- Article
- The development and application of a molecular community profiling strategy to identify polymicrobial bacterial DNA in the whole blood of septic patients.BMC microbiology · 2015Article
- Culture and molecular-based profiles show shifts in bacterial communities of the upper respiratory tract that occur with age.The ISME journal · 2015Article
- The potential use of bacterial community succession in forensics as described by high throughput metagenomic sequencing.International journal of legal medicine · 2014Article
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- Culture-independence for surveillance and epidemiology.Pathogens (Basel, Switzerland) · 2013Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polymicrobial nature of invasive pyogenic infections may be underestimated by routine culture practices, due to the fastidious nature of many organisms and the loss of viability during transport or from prior antibacterials. Pyrosequencing was performed on brain and liver abscesses and pleural fluid and compared to routine culture data. Forty-seven invasive pyogenic infection samples from 44 patients [6 intracerebral abscess (ICA), 21 pyogenic liver abscess (PLA), and 18 pleural fluid (PF) samples] were assayed. Pyrosequencing identified an etiologic microorganism in 100 % of samples versus 45 % by culture, p <0.01. Pyrosequencing was also more likely than traditional cultures to classify infections as polymicrobial, 91 % versus 17 %, p <0.001. The median number of genera identified by pyrosequencing compared to culture was 1 [interquartile range (IQR) 1-3] versus 0 (IQR 0-1) for ICA, 7 (IQR 1-15) versus 1 (IQR 0-1) for PLA, and 15 (IQR 9-19) versus 0 (IQR 0-1) for PF. Where organisms were cultured, they typically represented the numerically dominant species identified by pyrosequencing. Complex microbial communities are involved in invasive pyogenic infection of the lung, liver, and brain. Defining the polymicrobial nature of invasive pyogenic infections is the first step towards appreciating the clinical and diagnostic implications of these complex communities.
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