ArticleParasitology research2012
The use of different diagnostic tools for Babesia and Theileria parasites in cattle in Menofia, Egypt.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Status of babesiosis among domestic herbivores in Iran: a systematic review and meta-analysis.Parasitology research · 2017Pooled it
- Microscopy-PCR discordance and shifting epidemiology in South India: advancing diagnostic precision for control of morphologically similar bovine hemopathogens (Babesia, Theileria, Anaplasma) from a hospital-based study.Tropical animal health and production · 2026Article
- Molecular epidemiology and risk factors associated with Babesia bigemina infection in cattle.Scientific reports · 2026Article
- An updated assessment of the molecular prevalence and risk factors of Babesia infection among crossbred cattle: a diagnostic cross-sectional study.BMC veterinary research · 2026Article
- Detection and genotypes of piroplasms affecting ruminants in the New Valley Governorate, Egypt.BMC veterinary research · 2025Article
- Hemoparasitism in grazing cattle and risk factors associated with husbandry management in an endemic area of Eastern Colombia.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Molecular epidemiology of bovine theileriosis and genetic diversity ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Molecular detection and characterization ofParasite epidemiology and control · 2024Article
- Occurrence of Babesia and Anaplasma in ruminants from the Catimbau National Park, Semiarid Region of Northeast Brazil.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2024Article
- Molecular Detection and Identification of Piroplasm in Cattle from Kathmandu Valley, Nepal.Pathogens (Basel, Switzerland) · 2023Article
- Multiplex PCR for rapid differential diagnosis of co-prevalent species of Theileria (Theileria annulata and Theileria orientalis) in cattle.Parasitology research · 2023Article
- Tick-borne diseases in Egypt: A one health perspective.One health (Amsterdam, Netherlands) · 2022Review
- Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks.Pathogens (Basel, Switzerland) · 2022Article
- Molecular epidemiology and genetic characterization of Theileria orientalis in cattle.Tropical animal health and production · 2022Article
- Prevalence and risk factors associated with tropical theileriosis in Egyptian dairy cattle.Veterinary world · 2022Article
- Molecular Detection and Characterization ofMicroorganisms · 2022Article
- Seroprevalence and risk factors of tropical theileriosis in smallholder asymptomatic large ruminants in Egypt.Frontiers in veterinary science · 2022Article
- Serological Survey of Babesia bigemina and Babesia bovis in Cattle and Water Buffaloes from Menoufia Province, Egypt.Acta parasitologica · 2021Article
- Cross-sectional survey of cattle haemopathogens in Constantine, Northeast Algeria.Veterinary medicine and science · 2021Article
- Phylogenetic study ofVeterinary world · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine piroplasmosis is caused by tick-borne hemoprotozoans of the genera Babesia and Theileria and is the most prevalent in tropical and subtropical countries, causing a major economic impact worldwide. In the current study, a total of 405 cattle of different ages, sexes, and breeds were randomly sampled for surveying and diagnosis of babesiosis and theileriosis using three methods: direct microscopy (blood smears), indirect fluorescent antibody test (IFAT) and polymerase chain reaction (PCR). Giemsa-stained blood smears revealed that, out of 405 examined cattle, 33 (8.15 %) were infected with Babesia sp. and 65 (16.05 %) with Theileria sp. (total number of infected cattle was 98). Mixed infection was seen in 11 (2.72 %) animals. Moreover, application of the three diagnostic assays on 158 randomly sampled cattle indicated that 17 (10.76 %) and 33 (20.89 %) were positive for Babesia and Theileria spp. by the direct smear technique, 25 (15.82 %) and 33 (20.89 %) by IFAT (fluorescence was greenish yellow for Babesia and yellowish for Theileria), and 20 (12.66 %) and 38 (24.05 %) by PCR. Using primers specific for Babesia and Theileria spp., we found that diagnostic bands appeared at ~350 and ~370 bp, respectively indicating the presence of these piroplasms. Statistically, there was a non-significant difference of the positivity in response to the three techniques; thus, any of these methods can be described as useful for diagnosing blood parasites in both domesticated animals and birds. On the basis of the obtained results, it could be concluded that direct microscopy can be used in acute infections, whereas IFAT and PCR are useful in chronicity.
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