ArticleNature methods2012
A simple, versatile method for GFP-based super-resolution microscopy via nanobodies.
Article in Nature methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 263 papers.
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Who cites it
263 citing papers in PubMed, 575 citations in OpenAlex.
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- Super-resolution atlas of SARS-CoV-2 infection reveals protease-dependent organelle maturation, dsRNA landscapes, and intracellular structural proteins.Nature communications · 2026Article
- ROOT-ExM: Super-Resolution Imaging of Proteins inBio-protocol · 2026Article
- Stoichiometrically Defined Antibody-DNA Conjugates for Quantitative Super-Resolution Imaging.Nano letters · 2026Article
- Tiny tools closing the gap: nanobodies in research and therapy.Function (Oxford, England) · 2026Review
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Dynamic Binder Exchange Improves Protein Labeling Efficiency in DNA-PAINT up to 15-Fold.Angewandte Chemie (International ed. in English) · 2026Article
- There and back again: a cell biologist's journey from organelles to molecules.Biological chemistry · 2025Review
- Nanoscale spatiotemporal cluster analysis of expressed and endogenous proteins.Nature protocols · 2025Review
- Nanoscale architecture and dynamics of CaJournal of molecular and cellular cardiology plus · 2025Article
- Advanced optical microscopy methods forBiophysical reviews · 2025Review
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- Imaging Ligand-Receptor Interactions at Single-Protein Resolution with DNA-PAINT.Small methods · 2025Article
- Visualization of F-Actin Through Expansion Microscopy (ExM) with Trifunctional Linker-Conjugated Phalloidin.Bio-protocol · 2025Article
- Combining nanobody labeling with STED microscopy reveals input-specific and layer-specific organization of neocortical synapses.PLoS biology · 2025Article
- Microtubules as a versatile reference standard for expansion microscopy.Communications biology · 2025Article
- Deciphering respiratory viral infections by harnessing organ-on-chip technology to explore the gut-lung axis.Open biology · 2025Review
- In Vitro Functional Validation of an Anti-FREM2 Nanobody for Glioblastoma Cell Targeting.Antibodies (Basel, Switzerland) · 2025Article
203 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a method to use any GFP-tagged construct in single-molecule super-resolution microscopy. By targeting GFP with small, high-affinity antibodies coupled to organic dyes, we achieved nanometer spatial resolution and minimal linkage error when analyzing microtubules, living neurons and yeast cells. We show that in combination with libraries encoding GFP-tagged proteins, virtually any known protein can immediately be used in super-resolution microscopy and that simplified labeling schemes allow high-throughput super-resolution imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.