ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Microfibrous substrate geometry as a critical trigger for organization, self-renewal, and differentiation of human embryonic stem cells within synthetic 3-dimensional microenvironments.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- PET/EVOH nonwoven fabrics supportRegenerative therapy · 2026Article
- A chemically-defined plastic scaffold for the xeno-free production of human pluripotent stem cells.Scientific reports · 2022Article
- Mechanosensing of Mechanical Confinement by Mesenchymal-Like Cells.Frontiers in physiology · 2020Review
- Developing defined substrates for stem cell culture and differentiation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Cell type-specific extracellular matrix guided the differentiation of human mesenchymal stem cells in 3D polymeric scaffolds.Journal of materials science. Materials in medicine · 2017Article
- Optimization of Polymer-ECM Composite Scaffolds for Tissue Engineering: Effect of Cells and Culture Conditions on Polymeric Nanofiber Mats.Journal of functional biomaterials · 2017Article
- Optical High Content Nanoscopy of Epigenetic Marks Decodes Phenotypic Divergence in Stem Cells.Scientific reports · 2017Article
- Profiling stem cell states in three-dimensional biomaterial niches using high content image informatics.Acta biomaterialia · 2016Article
- Vascularization of three-dimensional engineered tissues for regenerative medicine applications.Acta biomaterialia · 2016Review
- Generation and transplantation of reprogrammed human neurons in the brain using 3D microtopographic scaffolds.Nature communications · 2016Article
- Design Principles for Engineering of Tissues from Human Pluripotent Stem Cells.Current stem cell reports · 2016Article
- Three-dimensional, soft neotissue arrays as high throughput platforms for the interrogation of engineered tissue environments.Biomaterials · 2015Article
- Actin grips: circular actin-rich cytoskeletal structures that mediate the wrapping of polymeric microfibers by endothelial cells.Biomaterials · 2015Article
- Organizational metrics of interchromatin speckle factor domains: integrative classifier for stem cell adhesion & lineage signaling.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Substrates for clinical applicability of stem cells.World journal of stem cells · 2015Review
- Production of human pluripotent stem cell therapeutics under defined xeno-free conditions: progress and challenges.Stem cell reviews and reports · 2015Review
- Signals from the surface modulate differentiation of human pluripotent stem cells through glycosaminoglycans and integrins.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Establishment of a three-dimensional culture system of gastric stem cells supporting mucous cell differentiation using microfibrous polycaprolactone scaffolds.Cell proliferation · 2014Article
- Controlled delivery of fibroblast growth factor-9 from biodegradable poly(ester amide) fibers for building functional neovasculature.Pharmaceutical research · 2014Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Substrates used to culture human embryonic stem cells (hESCs) are typically 2-dimensional (2-D) in nature, with limited ability to recapitulate in vivo-like 3-dimensional (3-D) microenvironments. We examined critical determinants of hESC self-renewal in poly-d-lysine-pretreated synthetic polymer-based substrates with variable microgeometries, including planar 2-D films, macroporous 3-D sponges, and microfibrous 3-D fiber mats. Completely synthetic 2-D substrates and 3-D macroporous scaffolds failed to retain hESCs or support self-renewal or differentiation. However, synthetic microfibrous geometries made from electrospun polymer fibers were found to promote cell adhesion, viability, proliferation, self-renewal, and directed differentiation of hESCs in the absence of any exogenous matrix proteins. Mechanistic studies of hESC adhesion within microfibrous scaffolds indicated that enhanced cell confinement in such geometries increased cell-cell contacts and altered colony organization. Moreover, the microfibrous scaffolds also induced hESCs to deposit and organize extracellular matrix proteins like laminin such that the distribution of laminin was more closely associated with the cells than the Matrigel treatment, where the laminin remained associated with the coated fibers. The production of and binding to laminin was critical for formation of viable hESC colonies on synthetic fibrous scaffolds. Thus, synthetic substrates with specific 3-D microgeometries can support hESC colony formation, self-renewal, and directed differentiation to multiple lineages while obviating the stringent needs for complex, exogenous matrices. Similar scaffolds could serve as tools for developmental biology studies in 3-D and for stem cell differentiation in situ and transplantation using defined humanized conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.