ArticleCurrent biology : CB2012
Localized products of futile cycle/lrmp promote centrosome-nucleus attachment in the zebrafish zygote.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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40 citing papers in PubMed, 79 citations in OpenAlex.
- The KASH protein UNC-83 differentially regulates kinesin-1 activity to control developmental stage-specific nuclear migration.Current biology : CB · 2025Article
- The Drosophila nucleoporin ELYS is required for parental chromosome arrangement at fertilization.G3 (Bethesda, Md.) · 2025Article
- CARF regulates the alternative splicing and piwi/piRNA complexes during mouse spermatogenesis through PABPC1.Acta biochimica et biophysica Sinica · 2024Article
- Maternal regulation of the vertebrate oocyte-to-embryo transition.PLoS genetics · 2024Article
- The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein.The Journal of cell biology · 2023Article
- The midbody component Prc1-like is required for microtubule reorganization during cytokinesis and dorsal determinant segregation in the early zebrafish embryo.Development (Cambridge, England) · 2023Article
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- The centriolar satellite protein Cfap53 facilitates formation of the zygotic microtubule organizing center in the zebrafish embryo.Development (Cambridge, England) · 2022Article
- The assembly of a noncanonical LINC complex in Saccharomyces cerevisiae.Current genetics · 2022Review
- The LINC Complex Assists the Nuclear Import of Mechanosensitive Transcriptional Regulators.Results and problems in cell differentiation · 2022Article
- Identification of maternal-effect genes in zebrafish using maternal crispants.Development (Cambridge, England) · 2021Article
- Structures of FHOD1-Nesprin1/2 complexes reveal alternate binding modes for the FH3 domain of formins.Structure (London, England : 1993) · 2021Article
- Article
- Two mechanisms drive pronuclear migration in mouse zygotes.Nature communications · 2021Article
- Structural Analysis of Different LINC Complexes Reveals Distinct Binding Modes.Journal of molecular biology · 2020Article
- Somatic cell nuclear transfer in non-enucleated goldfish oocytes: understanding DNA fate during oocyte activation and first cellular division.Scientific reports · 2019Article
- Coordination of Embryogenesis by the Centrosome in Drosophila melanogaster.Results and problems in cell differentiation · 2019Review
- Gamete Nuclear Migration in Animals and Plants.Frontiers in plant science · 2019Review
- Slow calcium waves mediate furrow microtubule reorganization and germ plasm compaction in the early zebrafish embryo.Development (Cambridge, England) · 2018Article
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2 authors at 1 institution in 1 country.
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Abstract
backgroundThe centrosome has a well-established role as a microtubule organizer during mitosis and cytokinesis. In addition, it facilitates the union of parental haploid genomes following fertilization by nucleating a microtubule aster along which the female pronucleus migrates toward the male pronucleus. Stable associations between the sperm aster and the pronuclei are essential during this directed movement.
resultsOur studies reveal that the zebrafish gene futile cycle (fue) is required in the zygote for male pronucleus-centrosome attachment and female pronuclear migration. We show that fue encodes a novel, maternally-provided long form of lymphoid-restricted membrane protein (lrmp), a vertebrate-specific gene of unknown function. Both maternal lrmp messenger RNA (mRNA) and protein are highly localized in the zygote, in a largely overlapping pattern at nuclear membranes, centrosomes, and spindles. Truncated Lrmp-EGFP fusion proteins identified subcellular targeting signals in the C terminus of Lrmp; however, endogenous mRNA localization is likely important to ensure strict spatial expression of the protein. Localization of both Lrmp protein and lrmp RNA is defective in fue mutant embryos, indicating that correct targeting of lrmp gene products is dependent on Lrmp function.
conclusionsLrmp is a conserved vertebrate gene whose maternally inherited products are essential for nucleus-centrosome attachment and pronuclear congression during fertilization. Precise subcellular localization of lrmp products also suggests a requirement for strict spatiotemporal regulation of their function in the early embryo.
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