ArticleDiagnostic microbiology and infectious disease2012
Development of a multiplex polymerase chain reaction assay for diarrheagenic Escherichia coli and Shigella spp. and its evaluation on colonies, culture broths, and stool.
Article in Diagnostic microbiology and infectious disease, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Understanding the etiology of diarrheal illness in Cambodia in a case-control study from 2020 to 2023.Gut pathogens · 2025Article
- Article
- In vitro evaluation of the anti-pathogenic activity of Okoubaka aubrevillei on the human gastrointestinal tract.Zeitschrift fur Gastroenterologie · 2021Article
- Considerations for using ETEC and Shigella disease burden estimates to guide vaccine development strategy.Vaccine · 2019Review
- Detection of common diarrhea-causing pathogens in Northern Taiwan by multiplex polymerase chain reaction.Medicine · 2018Observational
- The antimicrobial activity of probiotic bacteriaElectronic physician · 2018Article
- Prevalence and virulence gene profiling of enteroaggregative Escherichia coli in malnourished and nourished Brazilian children.Diagnostic microbiology and infectious disease · 2017Article
- Epidemiology of enteroaggregative Escherichia coli infections and associated outcomes in the MAL-ED birth cohort.PLoS neglected tropical diseases · 2017Article
- Survival capabilities of Escherichia coli O26 isolated from cattle and clinical sources in Australia to disinfectants, acids and antimicrobials.BMC microbiology · 2017Article
- Longitudinal Comparison of Antibiotic Resistance in Diarrheagenic and Non-pathogenic Escherichia coli from Young Tanzanian Children.Frontiers in microbiology · 2016Article
- Association Between Shigella Infection and Diarrhea Varies Based on Location and Age of Children.The American journal of tropical medicine and hygiene · 2015Article
- Microbiologic methods utilized in the MAL-ED cohort study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Article
- Rapid genetic typing of diarrheagenic Escherichia coli using a two-tube modified molecular beacon based multiplex real-time PCR assay and its clinical application.Annals of clinical microbiology and antimicrobials · 2014Article
- Epidemiology and clinical manifestations of enteroaggregative Escherichia coli.Clinical microbiology reviews · 2014Review
- Comparison of the BD MAX enteric bacterial panel to routine culture methods for detection of Campylobacter, enterohemorrhagic Escherichia coli (O157), Salmonella, and Shigella isolates in preserved stool specimens.Journal of clinical microbiology · 2014Article
- Extended spectrum betalactamase producing Enteroaggregative Escherichia coli from young children in Iran.Gastroenterology and hepatology from bed to bench · 2014Article
- Etiology of diarrhea in Bangladeshi infants in the first year of life analyzed using molecular methods.The Journal of infectious diseases · 2013Article
- Enteroaggregative Escherichia coli quantification in children stool samples using quantitative PCR.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2013Article
- Quantitative PCR for detection of Shigella improves ascertainment of Shigella burden in children with moderate-to-severe diarrhea in low-income countries.Journal of clinical microbiology · 2013Article
- The impoverished gut--a triple burden of diarrhoea, stunting and chronic disease.Nature reviews. Gastroenterology & hepatology · 2013Review
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Detection of diarrheagenic Escherichia coli (DEC) typically depends on identification of virulence genes from stool cultures, not on stool itself. We developed a multiplex polymerase chain reaction (PCR) assay that detects key DEC virulence genes (stx1, stx2, eae, bfpA, ipaH, LT, STh, aaiC, aatA). The assay involved a multiplex PCR reaction followed by detection of amplicon(s) using Luminex beads. The assay was evaluated on over 100 colony and broth specimens. We then evaluated the assay using DNA extracted from stool, colony pools, and Gram-negative broths, using stool spiked with known quantities of DEC. Performance of the assay on stool DNA was most quantitative, while stool broth DNA offered the lowest limit of detection. The assay was prospectively evaluated on clinical specimens in Tanzania. Stool DNA yielded higher sensitivity than colony pools compared with broth DNA as the standard. We propose using this assay to screen for DEC directly in stool or stool broths.
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